Kleckner and coworkers discovered a protein bound to the 5' termini of double-stranded breaks.
In their effort to purify and identify this protein, what did they do to amplify the signal (enrich the number of covalently bound complexes), and which protein did they ultimately purify?
What will be an ideal response?
Which of the following methods can be used to determine the position of nucleosomes in the genome?
A. RNA-Seq
B. Western blotting
C. ChIP-Seq
D. epigenetic immunoprecipitation
E. immunofluorescence
A recombinant DNA was constructed by inserting the DNA of interest into the AmpR gene of a pBR322 vector. The vector also contains a TetR gene that is not interrupted.
After introducing the recombinant DNA into bacterial cells, the resulting transformants were tested on a variety of media. Which of the following results would be expected?
A. growth on both ampicillin containing media and tetracycline containing media
B. growth on ampicillin but not tetracycline containing media
C. growth on tetracycline but not ampicillin containing media
D. no growth on either ampicillin or tetracycline containing media
E. growth only on media containing both antibiotics
Which of the following is not true of eukaryotic signal transduction pathways that result in the regulation of gene expression?
A. The pathway frequently begins with a signal molecule binding to a cell surface receptor.
B. The signal is transduced to the nucleus.
C. They generally involve the localization of a protein.
D. Signal molecules are always transported into the target cellular compartment.
Which of the following characteristics of group I intron splicing is not true of group II intron splicing?
A. Splicing occurs without ATP.
B. The first nucleophile is a 3' hydroxyl.
C. Catalysis does not require a protein.
D. Proteins mediate pre-RNA conformation in vivo.
Quick Reply
