RNA-Seq accomplishes all the following except:
A. measure gene expression.
B. measure levels of cellular RNA polymerase.
C. discover and annotate complete transcripts.
D. characterize alternative splicing and polyadenylation.
E. identify the location of introns.
What activity of Pol I makes it uniquely suited for its role in bacterial DNA replication?
What will be an ideal response?
Site-specific recombinases can be used:
A. to generate synthesis of multiple copies of plasmids.
B. to convert dimeric chromosomes to monomeric forms.
C. in the lysogenic pathway to circularize phage DNA.
D. to evade host immune systems by the process of phase variation.
E. All of the choices given are true.
The apparent Vmax of dihydropteroate synthetase will _____________ when sulfanilamide is present while the Km of this enzyme will ______________.
A. decrease / increase
B. not change / increase
C. decrease / decrease
D. not change / decrease
E. not change / not change
Which components are used by the RNA-Seq method when evaluating a transcriptosome?
A. mRNA, microarray, reverse transcriptase, cDNA, adapter DNA with primers, deoxynucleotides, DNA polymerase
B. mRNA, reverse transcriptase, cDNA, adapter DNA with primers, deoxynucleotides, DNA polymerase, 2D gel electrophoresis
C. mRNA, rRNA, reverse transcriptase, cDNA, adapter DNA with primers, deoxynucleotides, DNA polymerase, computer alignment
D. mRNA, reverse transcriptase, cDNA, adapter DNA with primers, deoxynucleotides, DNA polymerase, computer alignment
E. mRNA, reverse transcriptase, cDNA, adapter DNA with primers, deoxynucleotides, RNA polymerase, computer alignment
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