Which of those listed below is LEAST similar in what is measured and concluded?
A) fluorescence in situ hybridization
B) GFP fusion protein
C) Northern blot
D) RT-PCR
The principle behind a nucleic acid probe design is that the probe itself must contain
A) a key complementary part of the target gene sequence of interest.
B) all of the nucleotide sequence of the gene of interest to conclusively identify the gene.
C) an antibody to specifically bind to the gene of interest.
D) at least three separate complementary regions of the gene of interest.
A shuttle vector is most useful for
A) engineering a complete metabolic pathway.
B) identifying the localization of a protein.
C) knocking out a gene by cassette displacement.
D) making a foreign protein in a mammalian cell.
Which of those below is NOT an important consideration when designing a fusion protein construct?
A) Avoid hybridization of the fusion gene in the artificial construct.
B) Reading frame is the same for both the fusion gene and reporter gene.
C) Transcriptional start and stop signals are shared.
D) Translational start and stop signals are shared.
What makes eukaryotic transcripts easier to isolate than transcripts in bacteria?
A) Eukaryotic transcripts are not methylated but their genes are often methylated.
B) Larger transcript size in eukaryotes enables easy size-selection methods.
C) mRNA is polyadenylated in eukaryotes.
D) Transcripts are the most abundant RNAs in eukaryotes.
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