During elongation of a cell during binary fission, small gaps caused by transglycosylases are formed before cell membrane precursors can be inserted.
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Heterotrophic bacteria will run out of organic growth substrates in batch cultures but chemostats can provide constant nutrient source for them to grow.
Chemostats for photoautotrophic bacteria are not necessary to maintain them at a constant growth phase because a light can artificially be turned on constantly.
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Identifying bactoprenol inside the rare bacteria that lack peptidoglycan would be unexpected considering its role in membrane biosynthesis.
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By using a stain targeting living cells only, microscopy would be a better approach to enumerate the number of living cells in a soil sample compared to viability counts of serially diluted soil.
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For most purposes of studying bacterial isolates, viable counting usually gives accurate information about the number of active cells present in a culture volume.
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