physioex 9, exercise 12

I just realized my physioex 9.0 exercise 12 sheet is due today and I dont think I have time to finish it because I have a million other things I need to do. If anybody has any of the answers and could help I would greatly greatly appreciate it! I have some of them done but I really need to get the rest of it done. Im usually very good at staying on top of my work but this week has been hectic and this assignment completely slipped my mind! Here are the questions..

Activity 1
1. Describe the importance of the washing steps in the direct antibody fluorescence test.
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4. How would the results be affected if a negative control gave a positive result?

Activity 2
1. Describe how you were able to determine what antigen is in the unknown well.
2. Why does the precipitan line form?
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Activity 3
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Activity 4
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3. Briefly describe how the nitrocellulose strips were prepared before the patient samples were added to them.
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