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sila sila
wrote...
Posts: 474
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6 years ago
Two common methods for obtaining large quantities of a DNA fragment or gene of interest is by PCR or by DNA
  cloning (for example, construction of a DNA library). Explain one advantage of using PCR and one advantage of using
  DNA cloning in a living cell. What will be an ideal response?

Question 2

Some restriction enzymes create blunt DNA ends, without the complementary single-stranded overhangs of sticky ends. What is the advantage of using restriction enzymes that create sticky ends vs. blunt ends for creating recombinant
  DNA molecules? What will be an ideal response?
 
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Replies
wrote...
6 years ago
Answer to q. 1

Advantages of PCR:1.
Faster (lots of DNA obtained in a few hours).
2. More specific (the specific DNA fragment or gene of interest can be easily targeted for
amplification).
Advantages of DNA cloning:1.
The sequence of the DNA fragment or gene of interest must be know in order to construct
DNA primers.

Answer to q. 2

Since sticky ends are complementary, they will pair up easily, forming hydrogen bonds
between base pairs. This allows DNA ligase to create covalent bonds between each DNA
fragment. Blunt ends do not have complementary ends and therefore do not pair up easily.
sila Author
wrote...
6 years ago
This helps so much, thank you for responding so quickly...
wrote...
6 years ago
No worries, I was online and bored Grinning Face with Smiling Eyes
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