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Home Q & A Board Biology-Related Homework Help Biochemistry
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Shibe Shibe
wrote...
2 years ago Edited: 2 years ago, bio_man
Hello,

I’m completely stumped on how to use the Kd to gauge relative concentration of bound vs free protein. I was told something along these lines:

\[K_d = \frac{( [A] \times [B] )}{[A \cdot B]}\]

“If you have fixed amount of A, and is below Kd, there will be relatively little AB, and mostly free A”

How does this make sense conceptually? I have been trying trying to draw the scenario out or prove it to myself mathematically but I am getting nowhere. Can anyone please help me?
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#1
Educator
2 years ago
Hi Shibe

I'm not sure what's necessarily being asked here. I found a resource online that might shine some light, but again, without an explanation of what those variables mean, I can't help further.

https://ocw.mit.edu/courses/biology/7-51-graduate-biochemistry-fall-2001/lecture-notes/fa01lec07.pdf
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