Enzyme-Controlled Reaction Lab

1.Describe the relationship between substrate concentration and the initial reaction rate of an enzyme-catalyzed reaction. Is this a linear relationship? What happens to the initial reaction rate as substrate concentration increases?

2. What is the maximum initial reaction rate for this enzyme at pH 7?

3. Explain why the maximum initial reaction rate cannot be reached at low substrate concentrations.

4. What does your data indicate about the optimum pH level for this enzyme-catalyzed reaction?

5. Enzymes function most efficiently at the temperature of a typical cell, which is 37 degrees Celsius. Increases or decreases in temperature can significantly lower the reaction rate. What does this suggest about the importance of temperature-regulating mechanisms in organisms? Explain.