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Home Q & A Board Biology-Related Homework Help Cell Biology
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Astatine Astatine
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9 years ago
In the process of isolating DNA for transcription and translation, you digest the DNA with BamHI and ligate the gene into a plasmid vector. However, even though all of the controls work, no transcription is obtained using your fragment. Working backward, you note that restriction digestion has removed approximately 250 base pairs from the 5' end of the gene. It is possible that during restriction digestion 
A) the termination signal was removed. 
B) the RNA polymerase became inactivated. 
C) primase could not bind. 
D) the promoter was removed. 
E) none of the above
Textbook 
Becker's World of the Cell

Becker's World of the Cell


Edition: 8th
Authors:
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virionvirion
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9 years ago
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Astatine Author
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#2
9 years ago
Glad I joined this site, thank you for your help.
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#3
9 years ago
It's a tricky question, glad I was able to deliver.
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