× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
2
g
1
Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
New Topic  
mashu mashu
wrote...
Posts: 1
Rep: 0 0
10 years ago
Hello,

I have an exericise to solve at home:


What is the size of GLUT1 amplicon if we use the following PCR-primers for human
GLUT1? Please remember that we used human GLUT1 cDNA as a template.

GLUT1 sense: 5´AACTCTTCAGCCAGGGTCCAC 3´

GLUT1 antisense: 5´CACAGTGAAGATGATGAAGAC 3´

I tried to solve it myself but I don't know if the resul is correct.

I used NCBI BLAST and I've got 119bp

I used different site and I've got 503bp

Now I'm a bit confused, because I don't know which result is the correct one. If both are wrong then please give me an advice how to solve it  Slight Smile

Thanks in advance   Grinning Face
Read 489 times

Related Topics

New Topic      
Quick Reply
Bold
Italic
Underline
Strikethrough
Subscript
Superscript
Insert Math Equation
Font Name
Font Color
Align left
Center
Align right
Bullet list
Numbered list
Insert a horizontal rule
Insert a table
Insert a Quote
View source
Explore
Post your homework questions and get free online help from our incredible volunteers
  2187 People Browsing
Start New Topic Take the Tour CoursesNew Study Tools Topics Trending Browse by Textbook
Related Images
  
 299
  
 345
  
 11443