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Home Q & A Board Biology-Related Homework Help Genetics and Developmental Biology
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mashu mashu
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9 years ago
Hello,

I have an exericise to solve at home:


What is the size of GLUT1 amplicon if we use the following PCR-primers for human
GLUT1? Please remember that we used human GLUT1 cDNA as a template.

GLUT1 sense: 5´AACTCTTCAGCCAGGGTCCAC 3´

GLUT1 antisense: 5´CACAGTGAAGATGATGAAGAC 3´

I tried to solve it myself but I don't know if the resul is correct.

I used NCBI BLAST and I've got 119bp

I used different site and I've got 503bp

Now I'm a bit confused, because I don't know which result is the correct one. If both are wrong then please give me an advice how to solve it  Slight Smile

Thanks in advance   Grinning Face
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