Can a urine sample evaluate accurate vitamin deficiency in your body? What about Blood testing?

It can determine some deficiencies at least.  Not sure about all.  Since I'm pretty sure you'd need to visit a doctor to get it tested, you may as well just ask them.  I'm sure they'd recommend a good alternative if that's not the best route to take.

Bloodwork is needed.
Urine is not able to detect those levels.

Both urine and blood samples r great ways 2 figure out if u r healthy or lacking something your body needs.Urine is short term and blood test is long term.Both samples can tell u alot they both contain evidence of what has been passing through your body and they both can help a doctor understand how your body is functioning.A urine sample can help determine symptons such as stds,kidney stones,dehydration,infections of the bladder or kidneys where a blood sample may not.A blood sample can help determine other conditions such as diabetes,cholesterol,iron deficiency ,diseases like H.I.V. some cancers both samples are pretty important really.

A competent physician would most likely advise you to test both blood and urine and perhaps stool in order to determine best course of action

a blood test will be needed, specifically blood calcidiol.