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The three basic steps of polymerase chain reaction (PCR) are denaturation, primer annealing, and pri

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The three basic steps of polymerase chain reaction (PCR) are denaturation, primer annealing, and pri

The three basic steps of polymerase chain reaction (PCR) are denaturation, primer annealing, and primer extension. Which specification listed below is correct?
A) Primer extension—37° C when Taq polymerase is used
B) Primer design—oligonucleotide of 15-30 base pairs
C) Primer extension—10 minutes during each cycle
D) Denaturation—50° C for 5 minutes
E) Primer annealing—95° C for 1 minute

Textbook

Clinical Laboratory Immunology

Edition: 1^st

Authors: Mahon, Tice

Read 259 times

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