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SM4Shhh SM4Shhh
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11 years ago
How is the use of a bacterial plasmid to clone and sequence a human gene related to genetics?
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11 years ago
hey i'm studying that now too!

Bacterial plasmid is a vector that helps to introduce foreign gene into the host cell, which is bacteria in this case.


in ur question, human gene is ur gene of interest, u want to clone it. some general examples, people clone human genes that codes for insulin to mass produce large amounts of insulin to sell. their buyers will be people with diabetes, who are having problems in production of insulin for one of the types of diabetes.
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11 years ago
Bacteria, as you know, are asexual and go through binary fission. Their DNA, when splitting, duplicates an EXACT copy of itself. Bacteria have circular DNA. You take a sequence of a human DNA and stick it into the bacteria DNA, and the bacteria replicates, replicating the human DNA strand with it. You then can extract it using restriction enzymes and they go through this whole process; and then you can isolate the many copies of the human gene.

This is related to genetics because it deals with genetic sequence, codons, etc. For instance, e.coli can now be mass produced via this technique.
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