× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
g
3
3
2
J
2
p
2
m
2
h
2
s
2
r
2
d
2
l
2
a
2
Home Q & A Board Biology-Related Homework Help Cell Biology
New Topic  
Miguel2233 Miguel2233
wrote...
Posts: 14
Rep: 0 0
11 years ago
If we want to grow a bacterial clone with a plasmid that carries a desired DNA fragment inserted within the antibiotic ampicillin resistance gene, we should not grow it in a medium containing ampicillin.
Read 584 times
2 Replies

Related Topics

Replies
wrote...
#1
11 years ago
That is false.  A medium containing ampicillin would select for clones that contain the plasmid with the ampicillin resistance gene.
wrote...
#2
11 years ago
your question isn't too clear, but to get the correct clone of the PLASMID not the bacterium. you must cut out the desired DNA fragment using a specific restriction enzyme, then the plasmids should be put into DNA lygase and agar to allow the DNA fragment to join back into plasmids and more to grow via mitosis, now the agar plate shoudl be put into ampicillin to determine which of the cultures of plasmids have managed to fully recieve the ampicillin resistance gene
New Topic      
Quick Reply
Explore
Post your homework questions and get free online help from our incredible volunteers
  837 People Browsing
 125 Signed Up Today
Start New Topic Take the Tour CoursesNew Study Tools Topics Trending Browse by Textbook
Live Chat
Related Images
  
 3652
  
 976
  
 1153
Your Opinion
Where do you get your textbooks?
Votes: 328
Previous poll results: Which 'study break' activity do you find most distracting?