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Does the position of genes on a plasmid matter?

David

wrote...

Posts: 12

Rep:

11 years ago

Does the position of genes on a plasmid matter?

I have to answer questions for a bacterial transformation of pGLO. DOes the the position of these genes on the plasmid matter? please help, thanks

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Miguel2233

wrote...

#1

11 years ago

Yes, absolutely the position of genes in a plasmid matters. If you are cloning something into the reporter cassette encoding GFP, it must be cloned in the correct position to get the gene product produced. In pGLO, a bidirectional promoter drives the expression of both GFP and a gene for utilizing arabinose.

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