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Home Q & A Board Biology-Related Homework Help Cell Biology
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_biology _biology
wrote...
12 years ago
(a) One of the reasons for the use of plasmid cloning vectors is the ease with which they can be separated from the bacterial chromosomal DNA. Describe how this is achieved.

(b) What are the different methods for introducing a plasmid DNA into a bacterial
cell? Which of these gives the highest efficiency of transformation?

(c) What is a 2-plasmid expression vector?
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wrote...
#1
12 years ago
(a) This is usually achieved by precipitating chromosomal DNA from an alkaline lysate by neutralizing with potassium acetate.  Plasmid DNA remains soluble.

(b) Cells can be made competent by several different chemical treatments and incubated with plasmid DNA, or plasmid DNA can be introduced by electroporation, which gives the highest efficiency of transformation.

(c) Never heard of this, sorry!
wrote...
#2
Educator
9 years ago
Quote from: smack (12 years ago)
(c) Never heard of this, sorry!

Me neither... Confounded Face
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