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Home Q & A Board Laboratory Help Introductory Courses
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bugmenot1 bugmenot1
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11 years ago
You have 500ml of a 10x buffer stock. You need to make 500ml of 1x buffer to use for an experiment. How much concentrated buffer and water would you need to use?

Ahh, I keep getting like two different answers for this one.
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BuggerFormicBuggerFormic
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11 years ago
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#2
11 years ago
Imagine that you needed a litre 1x from 10x.  100 mls 10x + 900 mls water would give you the required dilution but this would be twice as much as you need so half the amounts: 50 mls 10x + 450 mls water gives you what you need.
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