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Home Q & A Board Laboratory Help Upper-Year Courses
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tonesmith tonesmith
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11 years ago
Why is it necessary to block unoccupied binding sites in the microtiter wells?
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11 years ago
Enzyme Linked Immuno Sorbant Assay (ELISA) is so sensitive because of the detection method, i.e. using antibody, and visual detection. A positive control is needed because of the relative selectivity of the antibody. It can always bind to other stuff and give artifactually high values.  Nonspecific, unoccupied binding sites in the microtiter plate (as well as other places) have to be blocked or they will give a signal as though they were the analyte of interest.
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