Upper-Year Courses

Method for preparing a wet mount

Method for preparing a wet mount

What's the detailed procedure for preparing a wet mount for observing plant stems?

Anonymous   455   1 Reply

duddy

Mastering Astronomy: If we measure a subatomic particle's position very precisely, then...

Mastering Astronomy: If we measure a subatomic particle's position very precisely, then...

I am currently taking astronomy, and I need help with answers for chapter s4, or simply understanding whats going on, please help!!

Here's an example of a question:If we measure a subatomic particle's position very precisely, the

pipp   3837   2 Replies

Anonymous

How does PCA reduce the dimension of a statistical problem? How do you select the reduced dimension

How does PCA reduce the dimension of a statistical problem? How do you select the reduced dimension

Principal Components Analysis is described as a method of 'dimension reduction'. How does PCA reduce the dimension of a statistical problem? How do you select the reduced dimension for your problem.

bio_man   864   3 Replies

bio_man

Human Anatomy Exercises

Human Anatomy Exercises

I am posting some of my completed exercises from the lab manual Human Anatomy Laboratory Manual with Cat Dissections (6th ed.) by Elaine Marieb and Susan J. Mitchell. I will warn you ahead of time some of the s

zanydelight69   2535   2 Replies

zanydelight69

Evolution: Finches and Evolution Lab

Evolution: Finches and Evolution Lab

Q1. Why are the birds discussed in this lab called Darwin's finches?
a)Charles Darwin named them Geospiza leodarwini in honor of his son Leonard.
b)They come from the Darwin archipelago off the coast of Ecuador.
c)Charles Darwin c

juno1973   6513   3 Replies

Katrina Salinas

Suntans and Sunburns A Case Study

Suntans and Sunburns A Case Study

A Painful Winter Break

     On the first day after arriving in Australia for Christmas vacation, a University of Niagara student plays out in the sun for six hours.  Later that night he notices that the skin on his trunk, le

ojsummer   4733   6 Replies

Hooray_Sports

How does an ELISA indirectly detect infection by a disease-causing agent?

How does an ELISA indirectly detect infection by a disease-causing agent?

This question was asked in a lab packet when profiling patients being tested for Lyme Disease.

nursing1   1036   2 Replies

Dionisio Alfredo Mendoza

Double checking bioinformatics post lab questions!

Double checking bioinformatics post lab questions!

Hello all! Today had my first lab in bioinformatics course and we used NCBI and things like  PubMed and Uniprot databases.

Have only 3 questions to double check

1) What is a “nuclear splice mRNA variant” ?I put its wher

Mateyman   365   4 Replies

duddy

Respiratory volumes

Respiratory volumes

Predictions
1. During exercise:
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2. During exercise:
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3. During exercise:
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4. During exercise:
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5. During exercise:
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Materials and Methods
1. Dep

Dreamalive   937   2 Replies

vaa_nessa

Human Anatomy Exercises Post 8

Human Anatomy Exercises Post 8

I am posting some of my completed exercises from the lab manual Human Anatomy Laboratory Manual with Cat Dissections (6th ed.) by Elaine Marieb and Susan J. Mitchell. I will warn you ahead of time some of the scan

zanydelight69   2176   1 Reply

ryann

Human Anatomy Exercises Post 7

Human Anatomy Exercises Post 7

I am posting some of my completed exercises from the lab manual Human Anatomy Laboratory Manual with Cat Dissections (6th ed.) by Elaine Marieb and Susan J. Mitchell. I will warn you ahead of time some of the scan

zanydelight69   2962   1 Reply

ryann

More Human Anatomy Exercises

More Human Anatomy Exercises

I am posting some of my completed exercises from the lab manual Human Anatomy Laboratory Manual with Cat Dissections (6th ed.) by Elaine Marieb and Susan J. Mitchell. I will warn you ahead of time some of the s

zanydelight69   1671   1 Reply

ryann

Which of the following is true of an X-linked gene, but not of a Y-linked gene?

Which of the following is true of an X-linked gene, but not of a Y-linked gene?

Which of the following is true of an X-linked gene, but not of a Y-linked gene?

luckyday   627   1 Reply

bolbol

How are human mitochondria inherited?

How are human mitochondria inherited?

How are human mitochondria inherited?

luckyday   384   1 Reply

bolbol

Which statement below correctly describes the results?

Which statement below correctly describes the results?

A white-eyed female Drosophila is crossed with a red-eyed male Drosophila. Which statement below correctly describes the results?

luckyday   467   1 Reply

bolbol

What name is given to the most common phenotype in a natural population?

What name is given to the most common phenotype in a natural population?

What name is given to the most common phenotype in a natural population?

luckyday   407   1 Reply

bolbol

When Thomas Hunt Morgan crossed his red-eyed F1 generation flies to each other, the F2 generation in

When Thomas Hunt Morgan crossed his red-eyed F1 generation flies to each other, the F2 generation in

When Thomas Hunt Morgan crossed his red-eyed F1 generation flies to each other, the F2 generation included both red- and white-eyed flies. Remarkably, all the white-eyed flies were male. What was the explanation for this result?

luckyday   426   1 Reply

bolbol

In humans, what determines the sex of offspring and why?

In humans, what determines the sex of offspring and why?

In humans, what determines the sex of offspring and why?

luckyday   429   1 Reply

bolbol

Expressing two different membrane proteins on the same promoter?

Expressing two different membrane proteins on the same promoter?

How could I generate a stable mammalian cell line that expresses two different membrane proteins on the same promoter? I want to know what strategies I could take.

anonyvous   1095   1 Reply

dtimmons95

Best host for transduction of the camR gene

Best host for transduction of the camR gene

You are given three different strains of S. enterica for a transduction experiment.  Strain 1 has a wild-type phenotype, strain 2 has a defect in oligosaccharide synthesis and strain 3 is defective in Histidine synthesis?   Which of these s

karmahone   742   2 Replies

bio_man

How to calculate conjugation efficiency and determine results of EMB plate for antibiotic ...

How to calculate conjugation efficiency and determine results of EMB plate for antibiotic ...

So we are conferring resistance between 2 unrelated species of bacteria by conjugation.  The donor bacteria is Pseudomonas strain(tet^R) and the recipient bacteria is E. Coli strain(tob^R).  So after conjugation we plated them on LB(rich pl

Moso13   758   3 Replies

bio_man

Does the pacinian corpuscle

Does the pacinian corpuscle

The olfactory receptor also contains a membrane protein that recognizes isoamyl acetate and, via several other molecules, transduces the odor stimulus into a receptor potential. Does the Pacinian corpuscle likely have this isoamyl acetate receptor pr

Charmon   568   1 Reply

bio_man

Delay Artefacts In Nerve Conduction Velocity

Delay Artefacts In Nerve Conduction Velocity

I was shown a demonstration on estimating peripheral nerve conduction velocity in a healthy subject and have been asked about to look up about delay artefacts which limits the accuracy of the values. There is apparently two of these but I have not be

Archer502   519   N/A

Archer502

How to read bands from PCR product agarose gel electrophoresis

How to read bands from PCR product agarose gel electrophoresis

I am not quite sure what to make of my results.. I would like to say lane 5, 6, 7 are uncut DNA. But Im not sure im right.

lexxi00   605   1 Reply

bio_man

Why dilute ligation mixture before transformation?

Why dilute ligation mixture before transformation?

I understand that diluting the ligation reaction mixture prior to transformation increases the transformation efficiency, but why is it that less DNA yields better results in this case?

adriata   502   2 Replies

psyche360

Formamide in hybridization buffer is w/v? or v/v?

Formamide in hybridization buffer is w/v? or v/v?

Greetings, good friday.

I have a question about hybridization buffer..

actually, you know, hybridization include salt(SSC), detergent(SDS), and formamide. but SSC is selling by stock sol(20X) so we inject when calculate diluted

Koreanraichu   686   1 Reply

doubleu

In microarray, target DNA are labeled?

In microarray, target DNA are labeled?

I know about microarray, after target DNA immobilized we inject labeled probe DNA..
I run this DNA both probe and target are oligonucleotides, but I cannot what are labeled.
only probe labeled and injected at hybridization step? or both p

Koreanraichu   622   1 Reply

thesurgeon

What purpose does the kaleidoscope marker serve in your coomassie-stained gel? If you were doing a ...

What purpose does the kaleidoscope marker serve in your coomassie-stained gel? If you were doing a ...

What purpose does the kaleidoscope marker serve in your coomassie-stained gel? If you were doing a western blot, how would the kaleidoscope maker be useful?

sunnysunsun96   1024   1 Reply

bio_man

(surface plasmon resonance) which method are helpful for reduce negative curve?

(surface plasmon resonance) which method are helpful for reduce negative curve?

1X tbs w/ sample
(1X TBS buffer : 90% DW+10% 10X TBS)

<1>
sample 100ul(diluted at DW until 1000X)
DW 800ul
10X TBS 100ul

it's my co-worker's opinion. he said "sample in DW, then when you

Koreanraichu   785   N/A

Koreanraichu

During chlorophyll biosynthesis, which wavelength of light, if at all, would produce the optimum ...

During chlorophyll biosynthesis, which wavelength of light, if at all, would produce the optimum ...

I am performing an experiment of chlorophyll biosynthesis, so had a question about whether during the reduction of the D ring by NADPH in chlorophyll biosynthesis, is the process affected by different wavelengths of light. It will also be very helpfu

jashdhill   1062   N/A

jashdhill

-20 microbial glycerol stocks

-20 microbial glycerol stocks

hello,

I am keeping microbial stocks in 25% glycerol at -20 (-80 not available) for a few months. but I am noticing that the stocks are remaining liquid. will this affect the viability of the cells ? if so what can I do without repeating t

tahah   1346   N/A

tahah

How can I make my own CRISPR CAS9?

How can I make my own CRISPR CAS9?

CRISPR CAS9 kits are just really too expensive for me to purchase.
How can I make my own CRISPR CAS9 to use?

bioflea   884   1 Reply

bio_man

Micronucleus testing, stain too dark?

Micronucleus testing, stain too dark?

Hello there!  First time posting. 

In any case, we did a micronucleus test on rat bone marrow in class this past week.  I followed the directions to a T, and timed my staining to the second, with my slide in the Wright st

Wifey2myWifey   845   N/A

Wifey2myWifey

Why heat can not reverse my formaldehyde cross-linking product

Why heat can not reverse my formaldehyde cross-linking product

I was trying to pull down some associated proteins with my bait protein by formaldehyde cross-linking. my bait protein is 3XFLAG-tagged. So my final step is to elute my proteins out by Low pH glycine. Then, the eluted protein was split into two sampl

wuchenggang   1538   N/A

wuchenggang

How should I choose a suitable secondary antibody?

How should I choose a suitable secondary antibody?

When I do ELISA, how should I choose a suitable secondary antibody? I know that I should consider about the host species of the primary antibody, but I'm not very clear.

whcaroline   1829   1 Reply

bio_man

Enzymes and ice bucket

Enzymes and ice bucket

We store enzymes in -20C.
As I know that it stores with glycerol, which prevent freezing of enzymes.
But why we also need to put it on the ice bucket when we are using it?

Retata   1091   1 Reply

psyche360

Trace element utilisation project.

Trace element utilisation project.

I have recently been set the task of carrying out a research project based on trace element utilisation. After countless hours reading into trace elements and how different species from the three kingdoms of life utilise these (transporter/user prote

PCRDON   1037   1 Reply

bio_man

Why does frequency of Transduction rise to maximum then decline

Why does frequency of Transduction rise to maximum then decline

did an experiment where we tried to transduce a mutant gene into E.coli and wanted to see the effect that increasing the volume of Lysate (including bacteriophage) had on the number of transductants. the number of transductants rose as the volume of

kakalaka1   2047   1 Reply

bio_man

Testing Cancer Cells ?

Testing Cancer Cells ?

Hi,

I would like to test cancer cells. Practical Tests.
I can imagine to run some tests from cancer cells of a mouse.
Isolating them in a petri dish and testing different substances and reactions on them.

Are

remoz   916   N/A

remoz

How does the electronegativity of a metal affect the activity of catalase?

How does the electronegativity of a metal affect the activity of catalase?

   Without any further ado, I have a problem concerning my lab experiment. The research question about the topic of enzymes with which I came up (the shortened version of it is the title) is the one that I found the most amusing and interesting. So I w

Malefactor   1091   N/A

Malefactor