Upper-Year Courses

Determining glycosylation, transmembrane proteins, and peripheral proteins

Determining glycosylation, transmembrane proteins, and peripheral proteins

A general summation of the question is as follows:

I am "performing" an analysis of membrane proteins based on the separation between aqueous and detergent phase (using Triton X-114). Membranes are made soluble with the detergen

montelena   1384   1 Reply

ThePsychic

Outbreak scenario as team microbiologist HELP

Outbreak scenario as team microbiologist HELP

An outbreak of an unidentified bacteria has occurred in a remote village. You have been
selected to travel on the investigative medical team as the microbiologist. You are
allowed to take 80 pounds of equipment. Your plane can only carry

heartly   1644   1 Reply

jazminemartin

how to interpret this temperature vs product graph

how to interpret this temperature vs product graph

Below is a copy of the graph they completed for that assay.  Without anything other than the graph in front of you, provide two reasonable explanations for what you see.  Be concise.

Our class did an experiment in which we observ

fighter1   1000   3 Replies

duddy

Pectinase Enzyme Assay

Pectinase Enzyme Assay

I am working on pectinase enzyme assay. I incubated 900 ul of substrate for 10 minutes in the water bath, followed by adding 2ml of DNSA reagent, then 100ul of enzyme extract added finally i read the absorbance @ 540 OD. However the values are high.

olij   576   1 Reply

duddy

Trans-acting or cis-acting mutation help.

Trans-acting or cis-acting mutation help.

My laboratory has identified a new OPERON in E. coli—the Jun operon. We have identified six genes in the operon: JunA, JunB, JunC, JunD, JunE & JunF. We have identified one mutation in the operon: JunA-. Describe the experiment you would do to de

Scubastevie00   1238   1 Reply

skip5284

What is the difference between Protein Arrays, ELISA , and Western Blot?

What is the difference between Protein Arrays, ELISA , and Western Blot?

and in what situation would you use each immunoassay?

wingnutta   512   1 Reply

AAquily

Bees

Bees

Squar root of 87?


How to desect a bee hive

Skylerprue   542   1 Reply

duddy

How can this problem be started? Serial diution problem using DNA stock solution

How can this problem be started? Serial diution problem using DNA stock solution

It says: 
You are given 10 µL of a DNA stock solution that has 1.0 µg target DNA/µL. The target DNA molecule is 2.9 kb in length.

Describe a set of serial dilutions that would yield a standard curve with the following concentrat

chapstick323   3556   6 Replies

duddy

HELP! how should i persuade this reviewer?

HELP! how should i persuade this reviewer?

To investigate the effect of xxxxx(treatment) on bone metabolism in tail- suspended rats, studies were carried out focusing on bone quality and the molecular mechanisms.


60 Female 8-week old Sprague Dawley rat were randomly selected

glory123   1338   4 Replies

savio

Is there an antibody to Complement C1s protein that binds cynomolgus C1s?

Is there an antibody to Complement C1s protein that binds cynomolgus C1s?

Hi, I'm looking for a C1s antibody that binds to cynomolgus monkey C1s protein.  All the ones I have tried bind to human C1s, but very weakly to cyno C1s.  Anyone know of one or more?  Thanks.

madamecurious   970   3 Replies

bio_man

How can bacteria that are nitrate reduction negative turn red immediately after

How can bacteria that are nitrate reduction negative turn red immediately after

How can bacteria that are nitrate reduction negative turn red immediately after the addition of zinc?
 
In the nitrogen reduction test, depending on the initial results following the addition of nitrate reagents A and B, you might have to a

doseofmegan   508   1 Reply

erighter16

Microbiology unknown help

Microbiology unknown help

17 possible tests i can run. I don't know where to start with the "flow of things". say if i get staph, what tests should I run to differentiate between the others? Is there an outli

asdzxc   465   1 Reply

padre

Calculating GFR

Calculating GFR

I need to calculate GFR using creatinine clearance and MDRD equation. All the data I have is two absorbances for a standard (start = .321) (stop = .358) and two absorbances for urine (start = .288) (stop = .456).  The urine volume was 230 mL.&nb

guitargirl0812   1046   N/A

guitargirl0812

How to separate bacteria from eukariotic grinded tissue in liquid medium?

How to separate bacteria from eukariotic grinded tissue in liquid medium?

I have a liquid medium which contains a grinded eukariotic tissue and the bacteria that I want to separate from it in order to perform a DNA extraction. As the tissue particles are quite small, I can't remove them manually.

How can I

elsoja   1174   8 Replies

MorgsMidae

3T3 l1 cell differentiation culture

3T3 l1 cell differentiation culture

the fat accumulation is not enough after differentiation culture, can someone share the experience the culture experience?

Jamesmy   956   9 Replies

bio_man

General and Special Senses Lab Help Please(Kober lab 4015)

General and Special Senses Lab Help Please(Kober lab 4015)

Hi all this is from the Kober Lab for A&P 2 or the bridge course. I don;t have a friend to do these things with, so I'm just researching for those. Once I finish, I plan on posting what I got, please do the same if you have this class. I neve

123

monroe025   11922   30 Replies

Nicoleg

Microbiology Virus Assay

Microbiology Virus Assay

there's three methods of virus assays that our prof wants us to learn:

1.)counting assay
2.)plaque assay
3.) infectious dose

i don't know the first one. i can find the final two on the internet but the first

iScience   486   1 Reply

duddy

How does dry needling differ from acupuncture? Is it increasingly popular in PT

How does dry needling differ from acupuncture? Is it increasingly popular in PT

I understand that dry needling is particularly for treatment of myofascial trigger points, but from what I understand, acupuncture is as well.

norm2020   736   2 Replies

norm2020

Molecular Biology

Molecular Biology

Why are onion cells more advantageous for GFP?

moderato   544   1 Reply

nelsonpitter

turbidity at 405nm

turbidity at 405nm

Hello everyone,
We are working on a hydrogel composed of collagen, and I am watching its formation using a plate reader using absorbance at 405nm. And I was wondering why is turbidity measured at this wavelength?
Thank you very much!

michal1020   698   1 Reply

padre

What are the limits to U.S. long term economic growth?

What are the limits to U.S. long term economic growth?

What are the limits to the U.S. long-term economic growth? Is there anything that our government can do to address these limits, or would it be a bad idea to try?

cactimedic235   3244   2 Replies

padre

Rotifers

Rotifers

Hello everyone

How could we use Rotifers in aquaculture? What are their potentials in the aquaculture field and approximately how much do they return in their revenues for an aquaculturing investing company? And how long would they nee

ehd123   720   5 Replies

padre

Help with Raoult's law

Help with Raoult's law

Could anyone please help me with the attached question?

ehd123   878   3 Replies

padre

super resolution microscopy

super resolution microscopy

what is the super resolution microscopy equation for resolution? pleases help.

fareh   533   1 Reply

zoeksyrianos

Calculating % Oxidation

Calculating % Oxidation

In the biochemistry experiment, I measured the absorbance value of metal solutions.

I understand the concept that lower number of absorbance, the more oxidation
But I need to show the graph, absorbance vs % oxdiation

I h

kayleigh   515   3 Replies

padre

Incubators (Contaminated vs Non contaminated)

Incubators (Contaminated vs Non contaminated)

Hello,

 

I have a question regarding incubators. Where I work, we segregate our so called contaminated work (intentionaly contaminated media/agar plates) and non contaminated work (product analysis wich are usualy exempt of bact

Aenhim   564   1 Reply

padre

Caffeine and LB Agar

Caffeine and LB Agar

I'm doing an experiment that requires me to plate caffeine.  At which point do I add the caffeine to the agar?  Thanks for any help

michaelc1982   656   1 Reply

padre

experiments in silico????

experiments in silico????

What is meant by in silico?

ehd123   498   1 Reply

padre

Lab Logs

Lab Logs

Hello everyone

I need to write a laboratory log. This is the first time I do this and I do not even know what it is or how we're supposed to write it. Could anyone please help and if possible show a sample?

Thank yo

ehd123   1036   7 Replies

padre

SDS Solution Preparation

SDS Solution Preparation

For some reason, something that never happened to me before, I am having problem with preparing an SDS solution. I require 20% SDS solution to prepare an SDS-PAGE gel but when dissolving 40 g in 200 mL, this seems impossible to prepare because it doe

micro   1269   1 Reply

barry

Hormonal levels in fish

Hormonal levels in fish

Im studying a snapper, Lutjanus peru for my masters thesis
i have a lot information about many things of the fish, im working with broodstock born in captivity.
im trying to find out its hormone levels (E2, T and 11KT) before, during

Daniel from BCS   631   1 Reply

lribanda

How are anti bodies fixated to ELISA wells?

How are anti bodies fixated to ELISA wells?

I would like to just know the general  procedure in which an antibody meant to catch the other antibody or the analite is fixated to the coating (and what the coating is made of ) in the ELISA well.
as they are given already made

Daniel from BCS   1377   3 Replies

biology101

Help to refine scientific dissertation topic on Honey Bees?

Help to refine scientific dissertation topic on Honey Bees?

I have a 5000-6000 word dissertation to write on any scientific topic of my choice, to which I choose to study Honey Bees or Bees in general. However, the more I research, the more information I find and the more difficult it is to refine my search t

dcgreen   475   1 Reply

tryingtostudy

Determining Disease

Determining Disease

How is a fruit disease determined? If there are abnormalities on fruits, are these simply cut, minced and plated?

micro   670   1 Reply

padre

Microbioloby case study question: Gram-positive, endospore-forming bacillius

Microbioloby case study question: Gram-positive, endospore-forming bacillius

B. Douglas is admitted to the hospital to receive IV antibiotics for treatment of folliculitis.  He began a second round of antibiotics a few days ago, this time ampicillin. Doug has had diarrhea, abdominal cramps and fever.  Blood tests re

adpeckham   759   1 Reply

padre

Gametosis?

Gametosis?

Whats the difference between male and female gametosis?

jaymelovee   919   3 Replies

zoeksyrianos

Can pGEM-T Easy Vector JM109 cells take up multiple copies of the Plasmid?

Can pGEM-T Easy Vector JM109 cells take up multiple copies of the Plasmid?

I am using JM109 cells and the pGEM-T easy vector system II to clone a mixed microbial community for sequencing and I need to know whether a sinle copy or multiple copies can be taken up by each competent cell because this will impact on my ability t

!JRon@n   873   N/A

!JRon@n

is someone kind enough to share the answers to PhysioEx 9.0 exercises 1-10? Pls.

is someone kind enough to share the answers to PhysioEx 9.0 exercises 1-10? Pls.

Is there someone kind enough to share the answers to PhysioEx 9.0 exercises 1-10? Pls and thank you.

pallakjj   934   3 Replies

padre

Is anyone kind enough to share the PHYSIOEX 9.0 answers?

Is anyone kind enough to share the PHYSIOEX 9.0 answers?

I don't have my physioex 9.0 cd because my classmate left it at home when i needed it today, so he couldnt give it to me. Tomorrow is the due date and i was assigned to answer EXERCISE 2 ACTIVITY 6, all i need are the values for the graphs. Plea

aobg   1727   2 Replies

Alyyyyyyy

Lab question

Lab question

Does anybody have PhysioEx 9.0 Exercise 3 activity 5?  

Thank you in advance..

yonose00   616   1 Reply

padre