Upper-Year Courses

10 years ago

Protein Concentration Adjustment

If I have a protein sample which after plotting a calibration curve I've determined to be say 20 ug/mL in concentration, how do you adjust such sample to a particular concentration e.g. 15 ug/mL? By simple proportion by adding distilled water or

micro 485 1 Reply

10 years ago

Data Normalization

I'm plotting a graph with one of the axis being concentration which has a range of 160 - 0.04 ppm and 0 ppm. How would you normalize the axis/data since log cannot be used (since I'm considering 0 concentration as well)?

micro 638 7 Replies

10 years ago

96 well plate cross contamination

I am using a 96 well plate to test antibiotic semsitivity if bacteria but after incubation, i found bacteria even in the control (i.e the one not containing bacteria / blank). I am suspecting that while incubation, the condensation that formed on the

micro 1141 3 Replies

10 years ago

Dissolving Antibiotics

If an antibiotic used to determine the MIC does not dissolve in water, what is the best way to get it in solution (assuming its in solid form)? In protocols it is stated to dissolve in 95% ethanol but that obviously wouldn't make sense to perfor

micro 984 5 Replies

10 years ago

Electrophoresis Artefact

I am performing SDS-PAGE for protein samples and am getting a dense line at the front (see image). Any suggestions why this is occurring?

[img width=350 height=186]http://s23.postimg.org/sj05cs0rv/Screen_Shot_201

micro 583 2 Replies

10 years ago

How to calculate the total energy in a venturi flume?

I am doing a lab report for university and it involves a venturi flume and broad-crested weir (2 separate experiments) and need to calculate the total energy upstream of the obstruction and same downstream to calculate the energy loss. However, I ha

irina 729 1 Reply

HTML

10 years ago

Which of the following situations delay the development of muscle fatigue the lo

Which of the following situations delay the development of muscle fatigue the longest?
Consistent interruptions of muscle stimulation
Prolonged muscle contraction
Repetitive stimulation of a muscle

lvn_409 3222 1 Reply

L8trH8tr

10 years ago

help posting lab results

okay so i am new to this I am trying to figure out how I can help. I have a bunch of the physioex 9.1 labs stuff and i'd like to share what I have, but I have no idea how to go about doing it? Can anyone help?

tpeppers 863 3 Replies

10 years ago

Statistical Analysis

I'm comparing the number of colonies formed of E. coli in different concentrations of caffeine. So how many are formed in a low amount, a medium amount and a large amount. What is the best statistical test to run for these results.

michaelc1982 477 1 Reply

jobob12345

10 years ago

SDS-PAGE Staining 'Problem'

I have run SDS-PAGE several times now and I'm getting pretty good resolution and visible bands however the background is remaining faintly stained which makes the contrast a bit poor when I manipulate the contrast of the scanned image. I'm

micro 610 3 Replies

10 years ago

Do the heart rate and amplitude always change together? Why or why not?

Hi,
I am doing a post lab on frog physiology of the heart.

I need help with this question:

Do the rate and amplitude always change together? Why or why not?

I know generally speaking, they change together.

willow25 1323 3 Replies

10 years ago

Can someone help me with my biochemistry lab question?

Can anyone please help me with these lab questions?
These are questions from the lab called: Identification of Serum Glcoprotein by Polyacrylamide Gel Electrophoresis and Albumin Western Blotting.

1. Describe the results of your Coom

Nancy1 1834 2 Replies

bio_man

10 years ago

Gel Problem

I tried to do an SDS-PAGE gel but after pouring the separation gel, this is what I got:

When I poured

micro 874 8 Replies

10 years ago

Sreening Genomic or cDNA libraries

I've purified a protein and I'm interested in identifying splicing patterns and promotor elements that contribute to expression of the RNA.

The following is the answer to this question, which I don't understand at all. Can

GwynethK 629 1 Reply

savio

10 years ago

Aggregation of Bacteria

Why does ammonium sulfate aggregate bacteria in the SAT (salt aggregation test)?

1 2

micro 1653 17 Replies

10 years ago

Washing Cell Pellet

When it comes to washing a bacterial cell pellet with saline or PBS, if the protocol doesn't specify the quantity, what's a typical reasonable quantity?

micro 5749 12 Replies

10 years ago

Caffeine experiment

I'm looking to set up an experiment to see the effects of caffeine on bacteria. I'm looking for some advice on experimental design and how to set it up.

michaelc1982 569 1 Reply

10 years ago

PBS vs. Sodium Phosphate Buffer

Is PBS and Sodium phosphate buffer the same?

micro 6887 5 Replies

duddy

10 years ago

Sterilizing Chemicals

When washing cells after centrifugation and such, does the chemical buffer/wash need to be autoclaved first? Do you have to autoclave each chemical that you add to a cell pellet? Can't it decompose on autoclaving?

micro 671 1 Reply

10 years ago

Bacteria Absorbance

If you need to monitor bacterial growth by UV absorbance at 600 nm, do you take a small quantity from the culture and measure its absorbance every few hours and then discard it? Also, say after 24 hrs incubation you need to adjust the absorbance to a

micro 1620 1 Reply

10 years ago

Specificity of linkages in Deoxy ribose and ribose sugar?

hi,
In Deoxy ribose sugar -OH group is present in 5' , 3' ,1' , so there are 3 propabillity for dehydration reaction with a phosporic acid. but phosphate is bound to 5' and 3' carbon alone. why?

Then why deoxy

barathvaj 762 1 Reply

10 years ago

Lysis buffer preparation

I am following a protein extraction protocol which suggests a lysis buffer with the following components:

- 140 mM NaCl
- 2.7 mM KCl
- 10 mM disodium hydrogen phosphate
- 1.8 mM potassium dihydrogen phosphate
- pH 7.3 (

micro 2491 12 Replies

Alexx

10 years ago

How can you design an experiment to show segregation of proteins in rafts?

This can be in a live or artificial cell

aefinan 1988 1 Reply

10 years ago

Bacterial Quantities

When a protocol instructs to transfer 10 mL of bacteria, what does it mean exactly? I imagine it is being assumed that bacteria is cultured in broth but then again, how is 10 mL a quantity for bacteria? Shouldn't the optical density be stated? I

micro 1126 9 Replies

10 years ago

PhysioEx 9.0 EXERCISE 1-2-5

I lost my book and need help in the meanwhile while the book and cd are sent again. Much appreciated.

Do Activity 1 (Simulating Dialysis or Simple Diffusion) in Exercise 1 (The Cell) in the PhysioEx lab book. Answer the following qu

J8415 1413 1 Reply

10 years ago

PhysioEx 9 - Exercise 10: Acid-Base Balance (Activity 1)

Exercise 10: Acid-Base Balance: Activity 1: Hyperventilation Lab Report
Pre-lab Quiz Results
You scored 100% by answering 4 out of 4 questions correctly.
1. The fastest compensatory mechanism for maintaining pH homeostasis in the human

seth121 52195 1 Reply

10 years ago

Percentage medium

A protocol requires "2% broth". Does this mean that after I prepare the broth solution as instructed by the supplier, I should treat this as 100% thus requring to dilute it down to 2% or am I simplifying things? Sorry if this is a stupid qu

micro 550 1 Reply

Alexx

10 years ago

Propagation and Transfection of BHK cells? Please help!

Hi all,

I'm going to transfect Baby Hamster Kidney Fibroblasts with an MMP9 expressing plasmid.

I wonder to ask what is the expected result of the 3 different transfection conditions and why? Confounded Face

(i.e. which conditio

uulou 1020 1 Reply

10 years ago

PHYSio

Which of the following situations delay the development of muscle fatigue the longest?

Can anyone help on this please.

aggie25101 735 1 Reply

nite_n_day

10 years ago

PhysioEx 9.0 Exercise 3 Activities 1-9

Hi all,

I am looking to cross check many answers for all of the Activity questions in exercise 3. I haven't answered these questions from activity 8.

8.1 If you added more sodium to the extracellular solution, could

tiffad16 22910 7 Replies

10 years ago

PhysioEx help please

Can anyone help with this PhysioEx exercise please???

PhysioEx 9.0 Exercise 5

Activity 3

1. Which is more likely to occur, a change in blood vessel radius or a change in blood vessel length? Why?

lovethelight 1275 2 Replies

10 years ago

PhysioEx 9.0 HELP

I need some help with my physioEx 9.0 exercise 5 please

Activity 5

1. Explain the effect of incresing the right flow tube radius on the flow rate, resistance, and pump rate?

2. Describe what the left and r

lovethelight 2591 4 Replies

10 years ago

PhysioEx 9.0 Help

Needing some help and would be very greatful

PhysioEx 9.0 Exercise 6

Activity 3

1. Explain the effect that decresing the temperature had on the frog heart? How do you think the human heart woul

lovethelight 1188 2 Replies

10 years ago

PhysioEx 9.0 Exercise 7 HELP

Can anyone please help me with my PhysioEx 9.0 Exercise 7?

Activity 1

2. What additional skeletal muscles are utilized in an ERV activity?

3. What was the FEV (%) at the initial radius of 5.00 mm?

lovethelight 1300 2 Replies

10 years ago

Physioex 9.0 help

Can anyone please help me with my PhysioEx 9.0 Exercise 7?

Activity 1

3. What was the FEV (%) at the initial radius of 5.00 mm?

4. What happened to the FEV (%) as the radius of the airways decreased?

lovethelight 1737 3 Replies

10 years ago

Physio 9.0 Exercise 7 HELP

Can anyone help with these??

PhysioEx 9.0 Exercise 7

Activity 2

Can anyone please help me with my PhysioEx 9.0 Exercise 7?

Activity 2

1. What lung values changed in the sp

lovethelight 957 4 Replies

10 years ago

Physio 9.0 Post-lab Exercise 2 Activity 6

Which of the following describes the relationship between length and tension?
Which of the following describes the relationship between length and tension?
As the muscle's resting length increases, the active force will increase.

penag93 1133 3 Replies

10 years ago

Physio 9.0 Post-lab Exercise 2 Activitiy 5

Part A
In this activity, why did the muscle force decrease over time?
In this activity, why did the muscle force decrease over time?
    Muscle fatigue developed.
    The stimulation frequency was decreased.
    Intracellular calci

penag93 1639 2 Replies