Upper-Year Courses

Animal-Derived Laboratory Reagents

Animal-Derived Laboratory Reagents

I have a question about animal-derived laboratory reagents and products. I’m working in a strictly in-vitro lab that focuses mainly on western blots and flow cytometry. I was wondering the cost to animal life associated with these reagents. These inc

southtowns18   1075   1 Reply

lvnorbust

Physiology

Physiology

Can somebody please explain why the latent period in a muscle twitch doesn't change with the increasing intensity of the stimulus being applied.

lovinlovely   1112   5 Replies

bio_man

Even More Human Anatomy Exercises

Even More Human Anatomy Exercises

I am posting some of my completed exercises from the lab manual Human Anatomy Laboratory Manual with Cat Dissections (6th ed.) by Elaine Marieb and Susan J. Mitchell. I will warn you ahead of time some of the s

zanydelight69   4386   12 Replies

rebeccasoung

How effective is gel electrophoresis at seperating DNA mixtures from two donors?

How effective is gel electrophoresis at seperating DNA mixtures from two donors?

As in, does it produce two distinct bands?

zzz1090   759   2 Replies

micko0317

What goes to the negative side in gel electrophoresis?

What goes to the negative side in gel electrophoresis?

What type of substances in general would be attracted to the negative side?

smjsa79   626   1 Reply

nutjob2011

what would cause the different migration rate between each wells in agarose gel electrophoresis?

what would cause the different migration rate between each wells in agarose gel electrophoresis?

I have been having this trouble. DNA sample travels at different rates between different wells but I know they have the same size. The wells toward the middle of the gel always travel slower and the bands across the gel look like a rainbow shape. It

flowe12   1461   N/A

flowe12

How does gel electrophoresis help determine relatedness in a species?

How does gel electrophoresis help determine relatedness in a species?

A. It matches mRNA.
 B. It shows mutations.
 C. It compares DNA.
 D. It predicts adaptations.

bug2012   2187   1 Reply

mickey27121

What permits gel electrophoresis to separate fragments of DNA?

What permits gel electrophoresis to separate fragments of DNA?

a.) Differeneces in the sizes of DNA fragments only.
b.) The number of the negative charges on the fragments only.
c.) The net charge (negative or positive) on the fragments only.
d.) The size and net charge (negative or positive) on the fragments.

bugbugs4   1218   1 Reply

NursingStud

Whats the difference between southern blotting analysis and Electrophoresis?

Whats the difference between southern blotting analysis and Electrophoresis?

Do they give different results?

flowe12   548   1 Reply

florrye

How do you explain the digestion in Gel Electrophoresis?

How do you explain the digestion in Gel Electrophoresis?

I need to do an essay for a lab and i wasnt there.

firas kilani   407   1 Reply

buffzyzz

What are the factors that affect the amount of diffusion in gel electrophoresis?

What are the factors that affect the amount of diffusion in gel electrophoresis?

Also, are diffusion effects responsible for the sometimes "smiling" bands, as well as bands spreading out?

zyxomma77   531   2 Replies

micike

How to read bands in agarose gel electrophoresis?

How to read bands in agarose gel electrophoresis?

I have a band that is between two bands of the ladder, how would I read that? For example one of my bands migrated to a position between 2000 and 1500 bp. Would I just say that the band that landed between those two ladder bands is 1750bp?

nursinghelp   1605   1 Reply

nursingneeded

The Essential Cosmic Perspective 6th Edition

The Essential Cosmic Perspective 6th Edition

Does anyone have the answers for Chapters 11-18.  Question:  When I search for the answer to my question I get the message Download PDF Creator, but nothing is there.

Am I doing something wrong?  HELP!!!!!1

chamrock   2581   1 Reply

bio_man

in maintaining zero order for substrate in enzyme assay, how much substrate must be placed?

in maintaining zero order for substrate in enzyme assay, how much substrate must be placed?

is it 1) 10km or 2)10km or more

we had a test just now and its worth 5 marks..sheesh!

Illuminatus   829   1 Reply

leroy3

Would Biuret be a good reagent for an assay that is looking for hemoglobin in urine?

Would Biuret be a good reagent for an assay that is looking for hemoglobin in urine?

Would Biuret be a good reagent for an assay that is looking for hemoglobin in urine?

Midnightcharm   618   1 Reply

datoledo88

How do you simply explain cell proliferation assay(MTT)?

How do you simply explain cell proliferation assay(MTT)?

I am working on whitebloodcell proliferation. how can i relate this to MTT? what criteria to use? Im confused

Asinense   590   2 Replies

irina

What are the most important controls for a CHIP assay?

What are the most important controls for a CHIP assay?

What are the most important controls for a CHIP assay?

asinger96   501   1 Reply

les4212

How do you interpret the spots in a CAT enzyme assay?

How do you interpret the spots in a CAT enzyme assay?

How do you interpret the spots in a CAT enzyme assay?

Asinense   618   1 Reply

les58mac

what is difference between an assay and a test?

what is difference between an assay and a test?

this was my viva question which i couldn't answer

tommytomz   450   1 Reply

irina

Equal concentrations of different proteins will have the same OD (absorbance) value for a Bradford assay?

Equal concentrations of different proteins will have the same OD (absorbance) value for a Bradford assay?

True or false?

LES   721   2 Replies

tommytoughnut

Can someone explain the AGID assay to me?

Can someone explain the AGID assay to me?

I am researching information for veterinarians on avian influenza and I am not clear on why you would perform an AGID instead of an ELISA.  I guess I do not really understand what the AGID test is and why it is optimal for this disease.
Hmmm, so why

Asinense   573   2 Replies

les4212

What is the chromophore in the Bradford protein assay?

What is the chromophore in the Bradford protein assay?

What is the chromophore in the Bradford protein assay?

LESHUDSON74   1978   1 Reply

asis

how can i calculate the molarity of Ammonia solution with a minimum assay of 25% and a molecular weight of 17?

how can i calculate the molarity of Ammonia solution with a minimum assay of 25% and a molecular weight of 17?

using the following reaction, am bit lost on how to calculate the molarity of NH4OH solution:
AL2(SO4)3+ 6 NH4OH = 2 AL(OH)3+ 3(NH4)2SO4
will greatly appreciate your help on how to calculate the molarity of the Solution to start working on the reacti

asing96   4321   2 Replies

Illuminatus

what exactly the assay of a chemical mean?

what exactly the assay of a chemical mean?

its written on the bottles of various acid in lab..what it means?

illsy   1442   2 Replies

micronat

How do you do a colorimetric MTT assay?

How do you do a colorimetric MTT assay?

For figuring out how many cell deaths have occured

Midnightcharm   467   1 Reply

datoledo88

What is a protocol for a standard curve for a glucose assay?

What is a protocol for a standard curve for a glucose assay?

What is a protocol for a standard curve for a glucose assay?

tommyd   1670   1 Reply

illkidxy4

Will a yeast two-hybrid assay work using an integral membrane protein as the 'bait'?

Will a yeast two-hybrid assay work using an integral membrane protein as the 'bait'?

I am thinking of setting up an experiment to look for interacting partners with a viral integral membrane protein. Will this work, or n\do I need to use just the N and C terminal tails without the TMD?

tommyt_   906   2 Replies

Illuminatus

Is there such a thing as a viral plaque assay that uses mammalian cells?

Is there such a thing as a viral plaque assay that uses mammalian cells?

Is there such a thing as a viral plaque assay that uses mammalian cells?

lepurplebird   469   1 Reply

Illuminatus

Why use naproxen as internal standard in ketorolac injection assay?

Why use naproxen as internal standard in ketorolac injection assay?

Why use naproxen as internal standard in ketorolac injection assay?

tommynkell   531   1 Reply

midnight1

in a fire assay for gold what is the best way to overcome the effect of ilmenite and iron in the crucible?

in a fire assay for gold what is the best way to overcome the effect of ilmenite and iron in the crucible?

in a fire assay for gold what is the best way to overcome the effect of ilmenite and iron in the crucible?

asing96   539   1 Reply

smokebomb13

can you give me suggestion to increase efficiency and effectifity in my chromatography assay?

can you give me suggestion to increase efficiency and effectifity in my chromatography assay?

can you give me suggestion to increase efficiency and effectifity in my chromatography assay?

Illuminatus   520   1 Reply

tommytoughnut

Alternative method to pull down assay for protein-protein interactions?

Alternative method to pull down assay for protein-protein interactions?

I was wondering what other methods exist in addition to GSP-fusion/pull down assay and mass spec. to determine interaction of one protein with the other.  I won't be able to perform crystallography, so that is not an option.

Is there anything else?

illicitf8   647   1 Reply

micro_2012

How does the assay detect the level of activity??

How does the assay detect the level of activity??

How does the assay detect the level of activity??

ilndrz   467   1 Reply

LES

How to accurately do Protein/Enzyme Assay Calculations?

How to accurately do Protein/Enzyme Assay Calculations?

This is for my Biochemistry of Macromolecules Lab.....
For determining enzyme concentration (umole/min) for M1, P1, P2, and S2 using the fact that 9OD=1 umole, does these calculations seem reasonable.
The OD's are: M1=.888, P1=.352, P2=.473, and S2=

irina   5941   1 Reply

illicitf8

What is an assay and in particular a protein assay?

What is an assay and in particular a protein assay?

What is an assay and in particular a protein assay?

LESHA   738   1 Reply

Asinense

alcohol dehydrogenase assay, how do you know when things go wrong?

alcohol dehydrogenase assay, how do you know when things go wrong?

the actual question reads:
how would you know if the reaction preparation of a alcohol dehydrogenase has been compromised?

lerado1480t   462   1 Reply

smn

What is the difference between the Assay and Purity ?

What is the difference between the Assay and Purity ?

What is the difference between the Assay and Purity ?

smn   788   1 Reply

ilndrz

What is the principle of biuret method in protein assay and its purpose?

What is the principle of biuret method in protein assay and its purpose?

What is the principle of biuret method in protein assay and its purpose?

lerado1480t   1085   1 Reply

midkidd

How long does incubation take for chemistry assay's on the Abbott axiom?

How long does incubation take for chemistry assay's on the Abbott axiom?

How long does incubation take for chemistry assay's on the Abbott axiom?

tommynkell   474   1 Reply

tommy_gun

Can you explain me about the Lowry assay that use for determininng protein?

Can you explain me about the Lowry assay that use for determininng protein?

Can you explain me about the Lowry assay that use for determininng protein?

les4212   471   1 Reply

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