Upper-Year Courses

PhysioEx 9.0 Exercise 9 HELP

PhysioEx 9.0 Exercise 9 HELP

I am struggling with this.  Can someone help please.  PhysioEx 9.0 Exercise 9

Activity 1

3.  Starting at the renal corpuscle, list the components of the renal tubule as they are encountered by filtrate?

lovethelight   2427   4 Replies

doseofmegan

HDL sup-populations: alpha and beta migratory, what does it mean?

HDL sup-populations: alpha and beta migratory, what does it mean?

I've been studying the structure and function of different types of HDL. Smaller discoidal HDL is often referred to as being pre-beta migratory, whereas larger spherical HDL is referred to as alpha migratory, what does the alpha/beta designator

Zaniar   559   2 Replies

Zaniar

E. Coli Culturing

E. Coli Culturing

It appears that E. coli is best cultured for protein analysis in LB. What do you think about using nutrient broth as an alternative?

micro   546   1 Reply

nite_n_day

what is the effect of temperature on heart rate?

what is the effect of temperature on heart rate?

Can someone discuss the importance of homeostasis in maintaining normal heart function? and what is the effect of temperature on heart rate?
I only got some answers to the importance of homeostasis in maintaining nornal body function but no idea

tonny   1108   2 Replies

robertling

The mass of a neutrino is?

The mass of a neutrino is?

The mass of a neutrino is

joao   553   1 Reply

gautcp

Use the following gene in protein synthesis:

Use the following gene in protein synthesis:

 Use the following gene in protein synthesis:
 TAGGACCATTTTAGCCCC
 o  Show the mRNA.
 o  Show the tRNA.
 o  Name the amino acids that will be placed in the polypeptide chain

help

domenicog   2773   1 Reply

duddy

Physioex9.0 help

Physioex9.0 help

Hello please help..exercise 7 in physioex9.0

 draw a spirogram that depicts a persons volumes and capacities before and during a significant cough?

Chart2 spirometry results

Suezan26   5387   1 Reply

bio_man

Physioex Exer 5 Activity 7 Help

Physioex Exer 5 Activity 7 Help

1.
How is the flow rate affected when the right flow tube radius is kept constant (at 3.0mm) and the left flow tube radius is modified (either up or down)?

Attempt:

-   If the modification is increase i

qPaladin   1106   3 Replies

Beagle-D

Cardiovascular Adjustments during Moderate Exercise

Cardiovascular Adjustments during Moderate Exercise

One of the adjustments to moderate exercise that my lab manual lists is:
➢   Compared to rest, ventricular relaxation (diastole) is greatly shortened in time whereas ventricular contraction (systole) is similar in duration.

How is this

qPaladin   569   1 Reply

savio

Physiology questions - Cardiovascular Dynamics

Physiology questions - Cardiovascular Dynamics

Help would be greatly appreciated.

   1. Do increase (or decreasE) blood flow to a particular body system (eg. digestive), would it be better to adjust HR or blood vessel diameter? Explain.

   2. Explain why altering blood vesel l

qPaladin   813   2 Replies

qPaladin

Biological vs. Technical replicates and data processing, so confused!

Biological vs. Technical replicates and data processing, so confused!

How do you combine data from biological replicates that contain technical replicates into one set of data that represents an average for a repeated experiment with a n of > 3?

Example: Say I have cancer line X and drug Y. What I do:

CityD   996   1 Reply

colleen

physio ex 9.0

physio ex 9.0

I am having a hard time with physioex 9.0. if someone can help that would be greatly appreciated.

johnvaughn   726   1 Reply

bio_man

discrepancy b/t lower and upper div courses

discrepancy b/t lower and upper div courses

why is it that all the stuff we learned in lower div courses are contradictory in upper div?

bobaholic   546   1 Reply

bio_man

Osmolarity of water in red blood cells?

Osmolarity of water in red blood cells?

I am confused on how osmolarity of cells with water would cause a cell to swell, shrink, or burst. I know that a cell has a solution concentration of 300 mosm. In a lab that was done, we analyzed the impact of a red blood cell in solutions containing

Violagirl   1641   1 Reply

bio_man

human population growth and theenvironment

human population growth and theenvironment

Describe historical pattern of growth of the worldwide human population since our origin.

hootie   1014   1 Reply

MSC88

PhysioEx 9.0 Exercise 6

PhysioEx 9.0 Exercise 6

Anyone can help?  I would appreciate it

PhysioEx 9.0  Exercise 6

Activity 5

1.  Describe the efffect that increasing the calcium ions had on the heart?

2.  Describe the effect that incre

lovethelight   1191   1 Reply

robertling

Physio Ex 9.0 Exercise 6

Physio Ex 9.0 Exercise 6

HELP!!

PhysioEx 9.0  Exercise 6


Activity 4

1.  Describe the effect that pilocarpine had on the heart and why it had this effect? 

2.  Atropine is an acetylcholine antagonist? 

lovethelight   1113   1 Reply

robertling

PhysioEx 9.0 Exercise 6 help

PhysioEx 9.0 Exercise 6 help

Got some of this figured out but would appreciate some help.  Thank you !!

Activity 2

1.  Explain the effect that extreme vagus nerve stimulations had on the heart?

2.  Explain two ways that the heart c

lovethelight   1407   1 Reply

bio_man

Can someone define immortalized cell for me?

Can someone define immortalized cell for me?

HeLa cells are immortal, which mean they can live forever long, however, does it require feeding or freezing in order for it to stay alive? In class we're on the ELISA assay topic explaining how the HAT medium affects the myeloma cell and the normal

michiggy   959   1 Reply

SMOKEY2112

How exactly do they find levels of drugs and liver function in blood test?

How exactly do they find levels of drugs and liver function in blood test?

I can't find a clear answer online. All i see is, oh they take the blood, and from that the regular levels are blah blah blah....but what happens IN BETWEEN that. What instruments do they use to find those regular or non-regular levels.
I was just wo

michi   449   2 Replies

nursing 2012

Any body familiar with the Elisa test? Pls help?

Any body familiar with the Elisa test? Pls help?

In the ELISA test one usually test by groups of three.Why did you assay your samples in triplicate? thanks ahead of time

Rizzlefritz   456   2 Replies

riyaghuman

How do you test for the presence of antibodies in blood?

How do you test for the presence of antibodies in blood?

How quickly can they be detected in rabbits (how many test bleeds and how often?)
Do you remove the serum first and use column chromatography?

nursing   425   1 Reply

michelleyou

What is the best way to chemically separate two optical isomers?

What is the best way to chemically separate two optical isomers?

I am trying to find a currently used method,  but any suggestions are welcomed. The optical isomers are of a metal complex. Thanks.

riza   422   1 Reply

tonih79

How does a protein in buffer stick to a plastic plate?

How does a protein in buffer stick to a plastic plate?

In order to stick antibodies on to a plastic plate, we put the antibodies in a buffer and let it sit overnight at 4 C. I was wondering what the mechanism of this adhesion is, does it reach equillibrium? Is it east to get off if you wanted to remove i

rivertube   346   1 Reply

rizzy81

How can I improve my ELISA results for the cytokine m-IL12p70?

How can I improve my ELISA results for the cytokine m-IL12p70?

I have been doing an experiment with the cytokine m-IL12p70, and I was just wondering how I would improve the results of the experiment.  I know that there should be some kind of activity but everytime I do it specifically with IL12p70 it always come

Rizzlefritz   381   1 Reply

rizzy81

Why is the ELISA test so sensitive in detecting the presence of antibodies or antigens?

Why is the ELISA test so sensitive in detecting the presence of antibodies or antigens?

Why is the ELISA test so sensitive in detecting the presence of antibodies or antigens?

rizza mae   680   1 Reply

smluke2000

What is the purpose of using the secondary antibody in the ELISA test?

What is the purpose of using the secondary antibody in the ELISA test?

What is the purpose of using the secondary antibody in the ELISA test?

rizzlepuff8   1293   2 Replies

smmiller223

What is the use of positive and negative controls in elisa?

What is the use of positive and negative controls in elisa?

What is the use of positive and negative controls in elisa?

Rizzlefritz   540   1 Reply

micherrydun

do keratinocytes contain collagen and fibronectin?

do keratinocytes contain collagen and fibronectin?

I am doing an ELISA with keratinocytes. I want to measure collagen and fibronectin. However, I need to know if these keratinocytes make these things to begin with.

rixa85   374   1 Reply

tonigra

What is inhibition ELISA and how is it different from regular indirect ELISA? Like what are the basic steps?

What is inhibition ELISA and how is it different from regular indirect ELISA? Like what are the basic steps?

What is inhibition ELISA and how is it different from regular indirect ELISA? Like what are the basic steps?

toney32   575   1 Reply

illfavor

which type of elisa is popularly used?

which type of elisa is popularly used?

we are using types of elisa but i need to know that which type of elisa is mostly used

nursing123   392   1 Reply

smokeyd

Describe the mechanism of ELISA. Why is ELISA so sensitive? Why is it important to have a positive control?

Describe the mechanism of ELISA. Why is ELISA so sensitive? Why is it important to have a positive control?

Why is it necessary to block unoccupied binding sites in the microtiter wells?

tonesmith   4531   1 Reply

micherrydun

Can nucleic acids be detected by the ELISA format?

Can nucleic acids be detected by the ELISA format?

I was wondering if ELISA could detect nucleic acids and If so why? and if not, why not?

smm0309   534   2 Replies

rivers.butterfly

What is the purpose of incubation in ELISA?

What is the purpose of incubation in ELISA?

Also why are the plates read at 450nm?

smluke2000   772   1 Reply

rixter119

Why is the secondary antibody from the ELISA test conjugated with an enzyme?

Why is the secondary antibody from the ELISA test conjugated with an enzyme?

and what will happen when the enzyme and the substrate meet up?

illestb   538   2 Replies

smokebomb13

When doing an ELISA antibody test for anthrax, what reasons could there be for a positive test?

When doing an ELISA antibody test for anthrax, what reasons could there be for a positive test?

...if someone DOES NOT have the disease, and the test turns positive anyway, what are some reasons for this in terms of antibodies and antigens?

Also, why would you need to assay your samples in a triplicate and wash the testing wells after every st

RiverTmasco   450   1 Reply

illestchemist

Algebra problem, finding out the total amount of Elisa's paycheck...?

Algebra problem, finding out the total amount of Elisa's paycheck...?

Elisa got a paycheck from her part-time job. She spent some getting dinner, some on a movie, and some on clothes. She deposited the rest in her savings account. The total amount she spent was $148. The amount she saved was 20% more than the amount sh

irina   397   1 Reply

tonigra

What does Elisa readers read when we put the microplates in?

What does Elisa readers read when we put the microplates in?

what does the printed paper tell us?

illicitf8   442   1 Reply

smokebomb13

What is the difference between direct and indirect ELISA ?

What is the difference between direct and indirect ELISA ?

rizkallah15   626   1 Reply

smluke2000

Physioex 9.0 exercise 3 activity 6

Physioex 9.0 exercise 3 activity 6

Hi I am having a hard time in filling  out the activity chart on frequency of action potentials and finding out the activity questions.  Can anyone help who took this class.

LML9404   3636   4 Replies

ubeitgirl