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Home Q & A Board Laboratory Help Upper-Year Courses
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rivertube rivertube
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11 years ago
In order to stick antibodies on to a plastic plate, we put the antibodies in a buffer and let it sit overnight at 4 C. I was wondering what the mechanism of this adhesion is, does it reach equillibrium? Is it east to get off if you wanted to remove it without necessarily destroying it?
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11 years ago
If you are thinking about ELISA plates (96-well plates), then they are coated with a special chemical which become "active" at basic pH. If I am right, then you must use either Carbonate or Borate buffer, pH 9, to coat your proteins onto the plates. These buffers (and their pH) activate the compound which covers the plate and make it "stick" to the hydrophobic groups of your proteins. It is not specific and it may change the structure of some of the coated protein.
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