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RiverTmasco RiverTmasco
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11 years ago
...if someone DOES NOT have the disease, and the test turns positive anyway, what are some reasons for this in terms of antibodies and antigens?

Also, why would you need to assay your samples in a triplicate and wash the testing wells after every step?
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#1
11 years ago
1. They have been vaccinated for anthrax.

2. They have been exposed to the anthrax bacillus and have circulating antibodies to it; you don't need to have had the disease for this to occur.

3. They have had the disease, but so mildly they did not know it.  Common for dairy workers.

4. False positive due to binding of the primary antibody to a cross-reacting protein.

Triplicate assays--->better accuracy as random errors are averaged out.

Wash wells---> lower background noise from non-specific binding of the antibody in the assay.
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