× Didn't find what you were looking for? Ask a question
Top Posters
Since Sunday
5
a
5
k
5
c
5
B
5
l
5
C
4
s
4
a
4
t
4
i
4
r
4
New Topic  
GwynethK GwynethK
wrote...
Posts: 2
Rep: 0 0
10 years ago
I've purified a protein and I'm interested in identifying splicing patterns and promotor elements that contribute to expression of the RNA.

The following is the answer to this question, which I don't understand at all. Can someone better explain this?

Obtain protein sequence, make degenerate oligonucleotide, use oligo to screen a genomic library, obtain a clone with DNA that would encode protein (check by sequencing the DNA). Sequence will give you info about promoter and possible splicing patterns of the RNA. Could use the DNA to probe for RNA sequences (northern blot), or to screen a cDNA library (sequence appropriate clones which would represent the RNA sequences).

Read 630 times
1 Reply

Related Topics

Replies
wrote...
10 years ago
Could you update us on what you ended up doing?

Sorry for replying so late
Biology - The only science where multiplication and division mean the same thing.
New Topic      
Explore
Post your homework questions and get free online help from our incredible volunteers
  1328 People Browsing
Related Images
  
 37
  
 143
  
 23
Your Opinion
What's your favorite coffee beverage?
Votes: 274

Previous poll results: Who's your favorite biologist?