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Producing cDNA from mRNA
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2. Add reverse lranscrlpmse lo I l l I I TTTTT 5' synlhasize a strand 0' cDNA Poly-A tail I I I I I IIIII 5' Double-stranded 3. Panially digas! RNA DNA/RNA duplex with RNase H V 5'11'r      3’ 3,|IllI|||lIII|IllI|||lIl|||Ill|||llll|lIlI5r ITTTT 4.Add DNApalymeras-Ito synthesixe second slrand 0! DNA V S’mmmmmfi 3’ 37IIllIIIIIllIIIllIIlllllllll llllllllllllll57 ITTTT 5. Add DNA ligase to seal gaps V 5,   ’M‘W" I|lIII|I|Illl|lllI|l|llll|lIll|l|llll|lIll r 3 ITTTT5 Double-stranded cDNA
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