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michal1020 michal1020
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10 years ago
Hello everyone,
We are working on a hydrogel composed of collagen, and I am watching its formation using a plate reader using absorbance at 405nm. And I was wondering why is turbidity measured at this wavelength?
Thank you very much!
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Staff Member
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10 years ago
Not sure if this quote helps to answer the question:

"The presence of the various wavelengths can cause interference in the turbidity measurements as natural color and natural organic matter in the sample can absorb some specific wavelengths of light and reduce the intensity of the scattered light."

I did find a source for it, maybe you could be better understand than me.
Source  http://or.water.usgs.gov/grapher/fnu.html
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