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Scubastevie00 Scubastevie00
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10 years ago
My laboratory has identified a new OPERON in E. coli—the Jun operon. We have identified six genes in the operon: JunA, JunB, JunC, JunD, JunE & JunF. We have identified one mutation in the operon: JunA-. Describe the experiment you would do to determine whether JunA- is a Trans-acting Factor or a Cis-acting element?
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wrote...
10 years ago
PaJaMo experiment.

Constructing Partially Diploid cells in which the section of the chromosome from a wild type cell carrying the Jun genes was intorduced into cell carrying mutant version of Jun genes.

Now when the chromosomal genes are constitutively expressed,
In case of trans-acting factor : Because of a mutation in JunA gene, the wild type copy restores the actual function.
In case of cis-acting element : mutation causing constitutive expression in Jun operator, it could not be complemented by trans factor. The operator functions only in cis.
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