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asdfghjkl22222 asdfghjkl22222
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7 years ago
My laboratory has identified a new OPERON in E. coli—the Jun operon. We have identified six genes in the operon: JunA, JunB, JunC, JunD, JunE & JunF. We have identified one mutation in the operon: JunA-. Describe the experiment you would do to determine whether JunA- is a Trans-acting Factor or a Cis-acting element?
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Educator
7 years ago
Complementation test which is also known as cis-trans test can be used to determine whether JunA is a trans acting factor or a cis acting element.Through complementation test, different elements are added to the mutant and its effects on regulation can be studied.If Jun A is the promoter or the operator gene , then no transcription will occur which means that JunA is a cis acting element because promoter and operator in lac operon are always cis acting.If JunA is encodes a repressor protein and that can cause transcription to occur, that means it is trans acting element as repressor diffuses through the cell and binds to the DNA.
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