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iScience iScience
wrote...
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10 years ago
there's three methods of virus assays that our prof wants us to learn:

1.)counting assay
2.)plaque assay
3.) infectious dose

i don't know the first one. i can find the final two on the internet but the first one i couldn't even understand when i found it. can someone help me?

thanks
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wrote...
Staff Member
10 years ago
Hello, I found this on Wikipedia, so I hope it helps.

A cell-counting assay may determine the number of living cells, the number of dead cells, or the ratio of one cell type to another, such as numerating and typing red versus different types of white blood cells. This is measured by different physical methods (light transmission, electric current change). But other methods use biochemical probing cell structure or physiology (stains). Another application is to monitor cell culture (assays of cell proliferation or cytotoxicity). A cytotoxicity assay measures how toxic a chemical compound is to cells.
Source 
http://www.wikiprotocols.org/protocols/sybr-green-i-cell-counting-assay--sds-method-with-96-well-plates/10283
- Master of Science in Biology
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