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Home Q & A Board Laboratory Help Upper-Year Courses
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anonyvous anonyvous
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7 years ago
How could I generate a stable mammalian cell line that expresses two different membrane proteins on the same promoter? I want to know what strategies I could take.
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#1
7 years ago
Since you wish to express two gene under the same promoter, you can use an IRES sequence, or other such elements, between the genes to create a bicistronic gene. Alternatively, you can use a vector containing a bidirectional promoter, such as the pBI backbone. In this case you clone each gene into the backbone separately.
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