Upper-Year Courses

How does a protein in buffer stick to a plastic plate?

How does a protein in buffer stick to a plastic plate?

In order to stick antibodies on to a plastic plate, we put the antibodies in a buffer and let it sit overnight at 4 C. I was wondering what the mechanism of this adhesion is, does it reach equillibrium? Is it east to get off if you wanted to remove i

rivertube   346   1 Reply

rizzy81

Double checking bioinformatics post lab questions!

Double checking bioinformatics post lab questions!

Hello all! Today had my first lab in bioinformatics course and we used NCBI and things like  PubMed and Uniprot databases.

Have only 3 questions to double check

1) What is a “nuclear splice mRNA variant” ?I put its wher

Mateyman   349   4 Replies

duddy

Help with DIY Biohacking !?

Help with DIY Biohacking !?

Hi,
I love biotechnology especially Biohacking with home made biotech equipment. I get lab protocols from Openwetware.com and built my equipment with instructions from Hacketeria.com except the PCR Machine which i had shipped from openPCR. And for th

oapplesauce   371   1 Reply

teqeishajohnso

do keratinocytes contain collagen and fibronectin?

do keratinocytes contain collagen and fibronectin?

I am doing an ELISA with keratinocytes. I want to measure collagen and fibronectin. However, I need to know if these keratinocytes make these things to begin with.

rixa85   374   1 Reply

tonigra

How are human mitochondria inherited?

How are human mitochondria inherited?

How are human mitochondria inherited?

luckyday   375   1 Reply

bolbol

How can I improve my ELISA results for the cytokine m-IL12p70?

How can I improve my ELISA results for the cytokine m-IL12p70?

I have been doing an experiment with the cytokine m-IL12p70, and I was just wondering how I would improve the results of the experiment.  I know that there should be some kind of activity but everytime I do it specifically with IL12p70 it always come

Rizzlefritz   380   1 Reply

rizzy81

What name is given to the most common phenotype in a natural population?

What name is given to the most common phenotype in a natural population?

What name is given to the most common phenotype in a natural population?

luckyday   390   1 Reply

bolbol

which type of elisa is popularly used?

which type of elisa is popularly used?

we are using types of elisa but i need to know that which type of elisa is mostly used

nursing123   391   1 Reply

smokeyd

Algebra problem, finding out the total amount of Elisa's paycheck...?

Algebra problem, finding out the total amount of Elisa's paycheck...?

Elisa got a paycheck from her part-time job. She spent some getting dinner, some on a movie, and some on clothes. She deposited the rest in her savings account. The total amount she spent was $148. The amount she saved was 20% more than the amount sh

irina   396   1 Reply

tonigra

How do you explain the digestion in Gel Electrophoresis?

How do you explain the digestion in Gel Electrophoresis?

I need to do an essay for a lab and i wasnt there.

firas kilani   403   1 Reply

buffzyzz

When Thomas Hunt Morgan crossed his red-eyed F1 generation flies to each other, the F2 generation in

When Thomas Hunt Morgan crossed his red-eyed F1 generation flies to each other, the F2 generation in

When Thomas Hunt Morgan crossed his red-eyed F1 generation flies to each other, the F2 generation included both red- and white-eyed flies. Remarkably, all the white-eyed flies were male. What was the explanation for this result?

luckyday   413   1 Reply

bolbol

In humans, what determines the sex of offspring and why?

In humans, what determines the sex of offspring and why?

In humans, what determines the sex of offspring and why?

luckyday   414   1 Reply

bolbol

Method for preparing a wet mount

Method for preparing a wet mount

What's the detailed procedure for preparing a wet mount for observing plant stems?

Anonymous   419   1 Reply

duddy

What is the best way to chemically separate two optical isomers?

What is the best way to chemically separate two optical isomers?

I am trying to find a currently used method,  but any suggestions are welcomed. The optical isomers are of a metal complex. Thanks.

riza   422   1 Reply

tonih79

How do you test for the presence of antibodies in blood?

How do you test for the presence of antibodies in blood?

How quickly can they be detected in rabbits (how many test bleeds and how often?)
Do you remove the serum first and use column chromatography?

nursing   424   1 Reply

michelleyou

What does Elisa readers read when we put the microplates in?

What does Elisa readers read when we put the microplates in?

what does the printed paper tell us?

illicitf8   440   1 Reply

smokebomb13

what is difference between an assay and a test?

what is difference between an assay and a test?

this was my viva question which i couldn't answer

tommytomz   447   1 Reply

irina

Which statement below correctly describes the results?

Which statement below correctly describes the results?

A white-eyed female Drosophila is crossed with a red-eyed male Drosophila. Which statement below correctly describes the results?

luckyday   447   1 Reply

bolbol

When doing an ELISA antibody test for anthrax, what reasons could there be for a positive test?

When doing an ELISA antibody test for anthrax, what reasons could there be for a positive test?

...if someone DOES NOT have the disease, and the test turns positive anyway, what are some reasons for this in terms of antibodies and antigens?

Also, why would you need to assay your samples in a triplicate and wash the testing wells after every st

RiverTmasco   449   1 Reply

illestchemist

How exactly do they find levels of drugs and liver function in blood test?

How exactly do they find levels of drugs and liver function in blood test?

I can't find a clear answer online. All i see is, oh they take the blood, and from that the regular levels are blah blah blah....but what happens IN BETWEEN that. What instruments do they use to find those regular or non-regular levels.
I was just wo

michi   449   2 Replies

nursing 2012

I am getting my bands at the wrong location (further down in the gel) for all of the antibodies I use?

I am getting my bands at the wrong location (further down in the gel) for all of the antibodies I use?

I have been making lysates from rat epididymal adipocytes for 3 months. The protocol that I am using has been used in our lab for the past 6 years and it always worked. I have changes the lysis buffer and everything else to make sure that's not the p

oblinger   454   1 Reply

obamanizer

Any body familiar with the Elisa test? Pls help?

Any body familiar with the Elisa test? Pls help?

In the ELISA test one usually test by groups of three.Why did you assay your samples in triplicate? thanks ahead of time

Rizzlefritz   455   2 Replies

riyaghuman

How do you correct an Assay run on a GC for water?

How do you correct an Assay run on a GC for water?

its a GC with an FID.  I used to do this when i worked for a Chemical company i just dont remember how

MicroMan84   457   2 Replies

asinger96

alcohol dehydrogenase assay, how do you know when things go wrong?

alcohol dehydrogenase assay, how do you know when things go wrong?

the actual question reads:
how would you know if the reaction preparation of a alcohol dehydrogenase has been compromised?

lerado1480t   461   1 Reply

smn

Microbiology unknown help

Microbiology unknown help

17 possible tests i can run. I don't know where to start with the "flow of things". say if i get staph, what tests should I run to differentiate between the others? Is there an outli

asdzxc   465   1 Reply

padre

How does the assay detect the level of activity??

How does the assay detect the level of activity??

How does the assay detect the level of activity??

ilndrz   466   1 Reply

LES

How do you do a colorimetric MTT assay?

How do you do a colorimetric MTT assay?

For figuring out how many cell deaths have occured

Midnightcharm   466   1 Reply

datoledo88

Is there such a thing as a viral plaque assay that uses mammalian cells?

Is there such a thing as a viral plaque assay that uses mammalian cells?

Is there such a thing as a viral plaque assay that uses mammalian cells?

lepurplebird   468   1 Reply

Illuminatus

Can you explain me about the Lowry assay that use for determininng protein?

Can you explain me about the Lowry assay that use for determininng protein?

Can you explain me about the Lowry assay that use for determininng protein?

les4212   470   1 Reply

!JRon@n

How long does incubation take for chemistry assay's on the Abbott axiom?

How long does incubation take for chemistry assay's on the Abbott axiom?

How long does incubation take for chemistry assay's on the Abbott axiom?

tommynkell   472   1 Reply

tommy_gun

Help to refine scientific dissertation topic on Honey Bees?

Help to refine scientific dissertation topic on Honey Bees?

I have a 5000-6000 word dissertation to write on any scientific topic of my choice, to which I choose to study Honey Bees or Bees in general. However, the more I research, the more information I find and the more difficult it is to refine my search t

dcgreen   475   1 Reply

tryingtostudy

Statistical Analysis

Statistical Analysis

I'm comparing the number of colonies formed of E. coli in different concentrations of caffeine. So how many are formed in a low amount, a medium amount and a large amount.  What is the best statistical test to run for these results.

michaelc1982   477   1 Reply

jobob12345

Protein Concentration Adjustment

Protein Concentration Adjustment

If I have a protein sample which after plotting a calibration curve I've determined to be say 20 ug/mL in concentration, how do you adjust such sample to a particular concentration e.g. 15 ug/mL? By simple proportion by adding distilled water or

micro   485   1 Reply

padre

Microbiology Virus Assay

Microbiology Virus Assay

there's three methods of virus assays that our prof wants us to learn:

1.)counting assay
2.)plaque assay
3.) infectious dose

i don't know the first one. i can find the final two on the internet but the first

iScience   486   1 Reply

duddy

Why dilute ligation mixture before transformation?

Why dilute ligation mixture before transformation?

I understand that diluting the ligation reaction mixture prior to transformation increases the transformation efficiency, but why is it that less DNA yields better results in this case?

adriata   491   2 Replies

psyche360

Explain the role of pyridine in the synthesis.

Explain the role of pyridine in the synthesis.

In another standard lab text, the experimental protocol for acetaminophen synthesis requires addition of pyridine (C5H5N) to the reaction mixture.  Explain the role of pyridine in the synthesis.

teresaroman   493   1 Reply

obamab

experiments in silico????

experiments in silico????

What is meant by in silico?

ehd123   498   1 Reply

padre

What are the most important controls for a CHIP assay?

What are the most important controls for a CHIP assay?

What are the most important controls for a CHIP assay?

asinger96   500   1 Reply

les4212

Delay Artefacts In Nerve Conduction Velocity

Delay Artefacts In Nerve Conduction Velocity

I was shown a demonstration on estimating peripheral nerve conduction velocity in a healthy subject and have been asked about to look up about delay artefacts which limits the accuracy of the values. There is apparently two of these but I have not be

Archer502   502   N/A

Archer502

Of the substances that diffused through the bag, did all of the molecules diffuse out?

Of the substances that diffused through the bag, did all of the molecules diffuse out?

We will dialyze a solution of glucose and starch to observe:
?? The directional movement of glucose and starch.
?? The effect of a selectively permeable membrane.
In this lab, we will be using an indicator to test for the presence of starch and gluco

tommy12   503   1 Reply

terramishu