Upper-Year Courses

For Cytogenetic cultures in buffalo (2N 50),having problem, blood co-agulates after just 1hour of setting up?

For Cytogenetic cultures in buffalo (2N 50),having problem, blood co-agulates after just 1hour of setting up?

In order to study the chromosomal abnormalities in our river buffalo (2N 50) I am trying to establish a protocol for reproducible results however, for the last four months I am encountering a problem that within 1 - 12 the hours cultured samples show

juliar33   508   1 Reply

JulieA

How can bacteria that are nitrate reduction negative turn red immediately after

How can bacteria that are nitrate reduction negative turn red immediately after

How can bacteria that are nitrate reduction negative turn red immediately after the addition of zinc?
 
In the nitrogen reduction test, depending on the initial results following the addition of nitrate reagents A and B, you might have to a

doseofmegan   508   1 Reply

erighter16

Calculating % Oxidation

Calculating % Oxidation

In the biochemistry experiment, I measured the absorbance value of metal solutions.

I understand the concept that lower number of absorbance, the more oxidation
But I need to show the graph, absorbance vs % oxdiation

I h

kayleigh   516   3 Replies

padre

can you give me suggestion to increase efficiency and effectifity in my chromatography assay?

can you give me suggestion to increase efficiency and effectifity in my chromatography assay?

can you give me suggestion to increase efficiency and effectifity in my chromatography assay?

Illuminatus   516   1 Reply

tommytoughnut

What is the difference between Protein Arrays, ELISA , and Western Blot?

What is the difference between Protein Arrays, ELISA , and Western Blot?

and in what situation would you use each immunoassay?

wingnutta   517   1 Reply

AAquily

What are the factors that affect the amount of diffusion in gel electrophoresis?

What are the factors that affect the amount of diffusion in gel electrophoresis?

Also, are diffusion effects responsible for the sometimes "smiling" bands, as well as bands spreading out?

zyxomma77   528   2 Replies

micike

Why use naproxen as internal standard in ketorolac injection assay?

Why use naproxen as internal standard in ketorolac injection assay?

Why use naproxen as internal standard in ketorolac injection assay?

tommynkell   531   1 Reply

midnight1

Can nucleic acids be detected by the ELISA format?

Can nucleic acids be detected by the ELISA format?

I was wondering if ELISA could detect nucleic acids and If so why? and if not, why not?

smm0309   532   2 Replies

rivers.butterfly

super resolution microscopy

super resolution microscopy

what is the super resolution microscopy equation for resolution? pleases help.

fareh   533   1 Reply

zoeksyrianos

in a fire assay for gold what is the best way to overcome the effect of ilmenite and iron in the crucible?

in a fire assay for gold what is the best way to overcome the effect of ilmenite and iron in the crucible?

in a fire assay for gold what is the best way to overcome the effect of ilmenite and iron in the crucible?

asing96   535   1 Reply

smokebomb13

Why is the secondary antibody from the ELISA test conjugated with an enzyme?

Why is the secondary antibody from the ELISA test conjugated with an enzyme?

and what will happen when the enzyme and the substrate meet up?

illestb   538   2 Replies

smokebomb13

What is the use of positive and negative controls in elisa?

What is the use of positive and negative controls in elisa?

What is the use of positive and negative controls in elisa?

Rizzlefritz   539   1 Reply

micherrydun

Whats the difference between southern blotting analysis and Electrophoresis?

Whats the difference between southern blotting analysis and Electrophoresis?

Do they give different results?

flowe12   544   1 Reply

florrye

E. Coli Culturing

E. Coli Culturing

It appears that E. coli is best cultured for protein analysis in LB. What do you think about using nutrient broth as an alternative?

micro   546   1 Reply

nite_n_day

Molecular Biology

Molecular Biology

Why are onion cells more advantageous for GFP?

moderato   546   1 Reply

nelsonpitter

discrepancy b/t lower and upper div courses

discrepancy b/t lower and upper div courses

why is it that all the stuff we learned in lower div courses are contradictory in upper div?

bobaholic   546   1 Reply

bio_man

Bees

Bees

Squar root of 87?


How to desect a bee hive

Skylerprue   546   1 Reply

duddy

Measuring Large-Scale mRNA/Protein Expression?

Measuring Large-Scale mRNA/Protein Expression?

For all of those that are well versed in biochemistry techniques I need some help in determining the ideal protocol for my research. So I am trying to determine a specific protease which degrades a receptor molecule that my lab studies but I am start

Julieoct   549   2 Replies

irina

Percentage medium

Percentage medium

A protocol requires "2% broth". Does this mean that after I prepare the broth solution as instructed by the supplier, I should treat this as 100% thus requring to dilute it down to 2% or am I simplifying things? Sorry if this is a stupid qu

micro   550   1 Reply

Alexx

Does the pacinian corpuscle

Does the pacinian corpuscle

The olfactory receptor also contains a membrane protein that recognizes isoamyl acetate and, via several other molecules, transduces the odor stimulus into a receptor potential. Does the Pacinian corpuscle likely have this isoamyl acetate receptor pr

Charmon   552   1 Reply

bio_man

The mass of a neutrino is?

The mass of a neutrino is?

The mass of a neutrino is

joao   553   1 Reply

gautcp

HDL sup-populations: alpha and beta migratory, what does it mean?

HDL sup-populations: alpha and beta migratory, what does it mean?

I've been studying the structure and function of different types of HDL. Smaller discoidal HDL is often referred to as being pre-beta migratory, whereas larger spherical HDL is referred to as alpha migratory, what does the alpha/beta designator

Zaniar   559   2 Replies

Zaniar

Incubators (Contaminated vs Non contaminated)

Incubators (Contaminated vs Non contaminated)

Hello,

 

I have a question regarding incubators. Where I work, we segregate our so called contaminated work (intentionaly contaminated media/agar plates) and non contaminated work (product analysis wich are usualy exempt of bact

Aenhim   564   1 Reply

padre

In microbiology, what does 'spoVM::spc' signify?

In microbiology, what does 'spoVM::spc' signify?

what does 'spoVM::spc' signify??
thanks:)

Asmaa   565   2 Replies

Fire_Fly

Cardiovascular Adjustments during Moderate Exercise

Cardiovascular Adjustments during Moderate Exercise

One of the adjustments to moderate exercise that my lab manual lists is:
➢   Compared to rest, ventricular relaxation (diastole) is greatly shortened in time whereas ventricular contraction (systole) is similar in duration.

How is this

qPaladin   569   1 Reply

savio

Caffeine experiment

Caffeine experiment

I'm looking to set up an experiment to see the effects of caffeine on bacteria.  I'm looking for some advice on experimental design and how to set it up.

michaelc1982   569   1 Reply

padre

independent/dependent variable in science problem?

independent/dependent variable in science problem?

As a check of their laboratory protocol for growing mouse embryos in vitro, a group of scientists want to compare the mean mass of 15 embryos grown for two weeks in their lab (see data below) to an average mass value of 75 µg that was obtained in sev

obamanizer   570   1 Reply

julius_6483

What is the concentration of the obtained DNA?

What is the concentration of the obtained DNA?

DNA Extraction protocol
(Procedure)


Method
Qiagen Easy DNA blood and Tissue Kit will be used.
1-Bring all reagents at room temperature
2-Prepare the reagents as mentioned in the handbook (done by the instructor)
3-Add 20 µl proteinase  K to the b

terminator   571   1 Reply

obb

Can someone explain the AGID assay to me?

Can someone explain the AGID assay to me?

I am researching information for veterinarians on avian influenza and I am not clear on why you would perform an AGID instead of an ELISA.  I guess I do not really understand what the AGID test is and why it is optimal for this disease.
Hmmm, so why

Asinense   572   2 Replies

les4212

What is inhibition ELISA and how is it different from regular indirect ELISA? Like what are the basic steps?

What is inhibition ELISA and how is it different from regular indirect ELISA? Like what are the basic steps?

What is inhibition ELISA and how is it different from regular indirect ELISA? Like what are the basic steps?

toney32   575   1 Reply

illfavor

Pectinase Enzyme Assay

Pectinase Enzyme Assay

I am working on pectinase enzyme assay. I incubated 900 ul of substrate for 10 minutes in the water bath, followed by adding 2ml of DNSA reagent, then 100ul of enzyme extract added finally i read the absorbance @ 540 OD. However the values are high.

olij   578   1 Reply

duddy

Electrophoresis Artefact

Electrophoresis Artefact

I am performing SDS-PAGE for protein samples and am getting a dense line at the front (see image). Any suggestions why this is occurring?

[img width=350 height=186]http://s23.postimg.org/sj05cs0rv/Screen_Shot_201

micro   583   2 Replies

micro

How do you simply explain cell proliferation assay(MTT)?

How do you simply explain cell proliferation assay(MTT)?

I am working on whitebloodcell proliferation. how can i relate this to MTT? what criteria to use? Im confused

Asinense   587   2 Replies

irina

How to read bands from PCR product agarose gel electrophoresis

How to read bands from PCR product agarose gel electrophoresis

I am not quite sure what to make of my results.. I would like to say lane 5, 6, 7 are uncut DNA. But Im not sure im right.

lexxi00   592   1 Reply

bio_man

In microarray, target DNA are labeled?

In microarray, target DNA are labeled?

I know about microarray, after target DNA immobilized we inject labeled probe DNA..
I run this DNA both probe and target are oligonucleotides, but I cannot what are labeled.
only probe labeled and injected at hybridization step? or both p

Koreanraichu   608   1 Reply

thesurgeon

SDS-PAGE Staining 'Problem'

SDS-PAGE Staining 'Problem'

I have run SDS-PAGE several times now and I'm getting pretty good resolution and visible bands however the background is remaining faintly stained which makes the contrast a bit poor when I manipulate the contrast of the scanned image. I'm

micro   610   3 Replies

padre

Would Biuret be a good reagent for an assay that is looking for hemoglobin in urine?

Would Biuret be a good reagent for an assay that is looking for hemoglobin in urine?

Would Biuret be a good reagent for an assay that is looking for hemoglobin in urine?

Midnightcharm   614   1 Reply

datoledo88

Lab question

Lab question

Does anybody have PhysioEx 9.0 Exercise 3 activity 5?  

Thank you in advance..

yonose00   616   1 Reply

padre

How do you interpret the spots in a CAT enzyme assay?

How do you interpret the spots in a CAT enzyme assay?

How do you interpret the spots in a CAT enzyme assay?

Asinense   617   1 Reply

les58mac

Which of the following is true of an X-linked gene, but not of a Y-linked gene?

Which of the following is true of an X-linked gene, but not of a Y-linked gene?

Which of the following is true of an X-linked gene, but not of a Y-linked gene?

luckyday   619   1 Reply

bolbol