Upper-Year Courses

Can pGEM-T Easy Vector JM109 cells take up multiple copies of the Plasmid?

Can pGEM-T Easy Vector JM109 cells take up multiple copies of the Plasmid?

I am using JM109 cells and the pGEM-T easy vector system II to clone a mixed microbial community for sequencing and I need to know whether a sinle copy or multiple copies can be taken up by each competent cell because this will impact on my ability t

!JRon@n   880   N/A

!JRon@n

Can someone define immortalized cell for me?

Can someone define immortalized cell for me?

HeLa cells are immortal, which mean they can live forever long, however, does it require feeding or freezing in order for it to stay alive? In class we're on the ELISA assay topic explaining how the HAT medium affects the myeloma cell and the normal

michiggy   959   1 Reply

SMOKEY2112

Can someone explain the AGID assay to me?

Can someone explain the AGID assay to me?

I am researching information for veterinarians on avian influenza and I am not clear on why you would perform an AGID instead of an ELISA.  I guess I do not really understand what the AGID test is and why it is optimal for this disease.
Hmmm, so why

Asinense   572   2 Replies

les4212

Can someone help me with my biochemistry lab question?

Can someone help me with my biochemistry lab question?

Can anyone please help me with these lab questions?
These are questions from the lab called: Identification of Serum Glcoprotein by Polyacrylamide Gel Electrophoresis and Albumin Western Blotting.

1. Describe the results of your Coom

Nancy1   1834   2 Replies

bio_man

Can someone help with Exercise 3 Activitys 6-9?

Can someone help with Exercise 3 Activitys 6-9?

I have been working on Exercise 3 for a while now and I first of all do know how to get these answers for the charts/tables and I also dk know how to know if they are right. If someone does not mind helping me out with the tables for Activities 6-9 i

awhitley001   1217   1 Reply

charlesisfat

Can someone help with PhysioEx Review 3?

Can someone help with PhysioEx Review 3?

I'd like to compare answers with someone on this section.

carabear   1296   2 Replies

sclowers

Can someone please help me with PhysioEx 8.0 exercise 8?

Can someone please help me with PhysioEx 8.0 exercise 8?

Can someone please help me with PhysioEx 8.0 exercise 8? (Human Physiology)  I specifically need help with activities 3, 4.  Of course, I'd be very happy with any help with any of those activities.  Thank so much!!

missbliss   4380   2 Replies

roxy

Can you explain me about the Lowry assay that use for determininng protein?

Can you explain me about the Lowry assay that use for determininng protein?

Can you explain me about the Lowry assay that use for determininng protein?

les4212   470   1 Reply

!JRon@n

can you give me suggestion to increase efficiency and effectifity in my chromatography assay?

can you give me suggestion to increase efficiency and effectifity in my chromatography assay?

can you give me suggestion to increase efficiency and effectifity in my chromatography assay?

Illuminatus   516   1 Reply

tommytoughnut

Cardiovascular Adjustments during Moderate Exercise

Cardiovascular Adjustments during Moderate Exercise

One of the adjustments to moderate exercise that my lab manual lists is:
➢   Compared to rest, ventricular relaxation (diastole) is greatly shortened in time whereas ventricular contraction (systole) is similar in duration.

How is this

qPaladin   569   1 Reply

savio

Cardiovascular Dynamics Physio Exercise 5

Cardiovascular Dynamics Physio Exercise 5

1. Describe the components in the blood that affect viscosity.

2. Describe the effect that radius changes have on the laminar flow of a fluid.

THANKS!!!

mrbarnhill   4830   2 Replies

bio_man

Cardiovascular Dynamics PhysioEx

Cardiovascular Dynamics PhysioEx

1. Which is more likely to occur, a change in blood vessel radius or a change in blood vessel length? Why?

2. Explain the effect that the change in blood vessel length had on flow rate.

mrbarnhill   4687   1 Reply

bio_man

Could anyone explain to me some basics on protein research protocols?

Could anyone explain to me some basics on protein research protocols?

I was moved to protein research lab recently but I don't have any experience in that feild.I was working in the Genomics dapartment before. So now I'm learning and practicing the techniques and protocols of acrylamide gel,staining...etc, but I can't

oapplesauce   640   2 Replies

julielpn74

Data Normalization

Data Normalization

I'm plotting a graph with one of the axis being concentration which has a range of 160 - 0.04 ppm and 0 ppm. How would you normalize the axis/data since log cannot be used (since I'm considering 0 concentration as well)?

micro   638   7 Replies

padre

Delay Artefacts In Nerve Conduction Velocity

Delay Artefacts In Nerve Conduction Velocity

I was shown a demonstration on estimating peripheral nerve conduction velocity in a healthy subject and have been asked about to look up about delay artefacts which limits the accuracy of the values. There is apparently two of these but I have not be

Archer502   503   N/A

Archer502

Describe the chemical changes that are occuring during this period?

Describe the chemical changes that are occuring during this period?

In activity 2, how long was the latent period? 2.78
-Describe the chemical changes that are occuring during this period?

From activity 3, describe the effect of the increasing voltage. What happened to the forst generated and why did

lil_one7979   7024   5 Replies

duddy

Describe the mechanism of ELISA. Why is ELISA so sensitive? Why is it important to have a positive control?

Describe the mechanism of ELISA. Why is ELISA so sensitive? Why is it important to have a positive control?

Why is it necessary to block unoccupied binding sites in the microtiter wells?

tonesmith   4531   1 Reply

micherrydun

Describe the significance of using 9mM sodium chloride inside the cell and 6 mM

Describe the significance of using 9mM sodium chloride inside the cell and 6 mM

I am struggling with my PhysioEx. 8.0.  On the review sheets for exercise 3. I am in need of help for the following questions. 4. What is the difference between membrane irritability and membrane conductivity? 5. Why does a nerve's action p

whitecaddy26   11752   1 Reply

boeing

Describe why Ringer’s solution is required to maintain heart contractions.

Describe why Ringer’s solution is required to maintain heart contractions.

1.   Which of the following types of muscle tissue can depolarize spontaneously in the absence of any external stimulation:
a.   Skeletal muscle
b.   Smooth muscle    
c.   Cardiac muscle
d.   All of the above
2.   Look at Figure 6.1, the

britni1186   7024   2 Replies

George

Describe your four tracings and explain what has happened in each of them.

Describe your four tracings and explain what has happened in each of them.

I am stuck on just a few questions and wondered if anyone could help me out.

Activity 3 question: An individual muscle fiber follows the all-or-none principle—it will either contract 100% or not at all.  Does the muscle we are working

sunshine   1599   N/A

sunshine

Design an experiment outlining your techniques that will analyze the temporal expression pattern of

Design an experiment outlining your techniques that will analyze the temporal expression pattern of

Design an experiment outlining your techniques that will analyze the temporal expression pattern of a gene (gene X - mRNA expression) and its protein localization during gastrulation in three different organisms.
Please state the organisms you a

gkor29   3129   N/A

gkor29

Determining Disease

Determining Disease

How is a fruit disease determined? If there are abnormalities on fruits, are these simply cut, minced and plated?

micro   670   1 Reply

padre

Determining glycosylation, transmembrane proteins, and peripheral proteins

Determining glycosylation, transmembrane proteins, and peripheral proteins

A general summation of the question is as follows:

I am "performing" an analysis of membrane proteins based on the separation between aqueous and detergent phase (using Triton X-114). Membranes are made soluble with the detergen

montelena   1386   1 Reply

ThePsychic

Did you get the flu shot?

Did you get the flu shot?

Czech newspapers are questioning if the shocking discovery of vaccines contaminated with the deadly avian flu virus which were distributed to 18 countries by the American company Baxter were part of a conspiracy to provoke a pandemic.
The claim holds

teribaer   700   2 Replies

davidc

Difficulty to come to a cunclusion from my SDS-PAGE gel electrophoresis results.

Difficulty to come to a cunclusion from my SDS-PAGE gel electrophoresis results.

In class I did a sds-page electrophoresis of e.coli expressed in four different sugars; Frucose, Arabinose, Glucose and Galactose, all in a concentration of 30 µl. When looking at my sds-page result, the four different samples seem to express the sam

Woahscience   2615   N/A

Woahscience

dihybrid cross with possible epistasis involved

dihybrid cross with possible epistasis involved

Using this data could you help me figure out if these genes are linked and if they are what’s the map distance between them? 
This is a dihybrid cross working with fruit flies.  Parental generation is a sepia eyed male x apterous fema

confused_student   4958   2 Replies

sarah!

discrepancy b/t lower and upper div courses

discrepancy b/t lower and upper div courses

why is it that all the stuff we learned in lower div courses are contradictory in upper div?

bobaholic   546   1 Reply

bio_man

Dissolving Antibiotics

Dissolving Antibiotics

If an antibiotic used to determine the MIC does not dissolve in water, what is the best way to get it in solution (assuming its in solid form)? In protocols it is stated to dissolve in 95% ethanol but that obviously wouldn't make sense to perfor

micro   984   5 Replies

padre

DNA fiber analysis protocol for s. pombe?

DNA fiber analysis protocol for s. pombe?

Hi,
Does anyone have experience on DNA fiber analysis (combing) for S. pombe organism? I need a thorough protocol for that if any one can spare one or leave a link to.

Thanks,
Parham

parham   757   2 Replies

duddy

do keratinocytes contain collagen and fibronectin?

do keratinocytes contain collagen and fibronectin?

I am doing an ELISA with keratinocytes. I want to measure collagen and fibronectin. However, I need to know if these keratinocytes make these things to begin with.

rixa85   374   1 Reply

tonigra

Do the heart rate and amplitude always change together? Why or why not?

Do the heart rate and amplitude always change together? Why or why not?

Hi,
I am doing a post lab on frog physiology of the heart.

I need help with this question:

Do the rate and amplitude always change together? Why or why not?

I know generally speaking, they change together.

willow25   1323   3 Replies

padre

does anyone has a good protocol to extract collagen type I from human/mamal bone

does anyone has a good protocol to extract collagen type I from human/mamal bone

 

nausicaa   8215   6 Replies

nausicaa

Does anyone have the blood lab review (not physioEx)?

Does anyone have the blood lab review (not physioEx)?

Hi, does anyone have the blood lab review from Elaine marieb katja Joehn, 8th edition. actually, any edition would work

caker   1036   1 Reply

bio_man

Does the pacinian corpuscle

Does the pacinian corpuscle

The olfactory receptor also contains a membrane protein that recognizes isoamyl acetate and, via several other molecules, transduces the odor stimulus into a receptor potential. Does the Pacinian corpuscle likely have this isoamyl acetate receptor pr

Charmon   552   1 Reply

bio_man

Double checking bioinformatics post lab questions!

Double checking bioinformatics post lab questions!

Hello all! Today had my first lab in bioinformatics course and we used NCBI and things like  PubMed and Uniprot databases.

Have only 3 questions to double check

1) What is a “nuclear splice mRNA variant” ?I put its wher

Mateyman   350   4 Replies

duddy

E. Coli Culturing

E. Coli Culturing

It appears that E. coli is best cultured for protein analysis in LB. What do you think about using nutrient broth as an alternative?

micro   546   1 Reply

nite_n_day

Electrophoresis Artefact

Electrophoresis Artefact

I am performing SDS-PAGE for protein samples and am getting a dense line at the front (see image). Any suggestions why this is occurring?

[img width=350 height=186]http://s23.postimg.org/sj05cs0rv/Screen_Shot_201

micro   583   2 Replies

micro

Enzyme for the synthesis of L-ascorbic acid

Enzyme for the synthesis of L-ascorbic acid

I am doing some university research which requires an enzyme that can produce L-ascorbic acid as the end product. The problem is that my knowledge of biology is limited and I have no idea if there is one available that does this. I have searched the

Goosebay   1171   1 Reply

bio_man

Enzymes and ice bucket

Enzymes and ice bucket

We store enzymes in -20C.
As I know that it stores with glycerol, which prevent freezing of enzymes.
But why we also need to put it on the ice bucket when we are using it?

Retata   1072   1 Reply

psyche360

Equal concentrations of different proteins will have the same OD (absorbance) value for a Bradford assay?

Equal concentrations of different proteins will have the same OD (absorbance) value for a Bradford assay?

True or false?

LES   719   2 Replies

tommytoughnut