Upper-Year Courses

The mass of a neutrino is?

The mass of a neutrino is?

The mass of a neutrino is

joao   553   1 Reply

gautcp

Why is the ELISA test so sensitive in detecting the presence of antibodies or antigens?

Why is the ELISA test so sensitive in detecting the presence of antibodies or antigens?

Why is the ELISA test so sensitive in detecting the presence of antibodies or antigens?

rizza mae   679   1 Reply

smluke2000

How can I improve my ELISA results for the cytokine m-IL12p70?

How can I improve my ELISA results for the cytokine m-IL12p70?

I have been doing an experiment with the cytokine m-IL12p70, and I was just wondering how I would improve the results of the experiment.  I know that there should be some kind of activity but everytime I do it specifically with IL12p70 it always come

Rizzlefritz   380   1 Reply

rizzy81

PhysioEx 9.0 Exercise 6 help

PhysioEx 9.0 Exercise 6 help

Got some of this figured out but would appreciate some help.  Thank you !!

Activity 2

1.  Explain the effect that extreme vagus nerve stimulations had on the heart?

2.  Explain two ways that the heart c

lovethelight   1407   1 Reply

bio_man

Can someone define immortalized cell for me?

Can someone define immortalized cell for me?

HeLa cells are immortal, which mean they can live forever long, however, does it require feeding or freezing in order for it to stay alive? In class we're on the ELISA assay topic explaining how the HAT medium affects the myeloma cell and the normal

michiggy   959   1 Reply

SMOKEY2112

How do you test for the presence of antibodies in blood?

How do you test for the presence of antibodies in blood?

How quickly can they be detected in rabbits (how many test bleeds and how often?)
Do you remove the serum first and use column chromatography?

nursing   425   1 Reply

michelleyou

What is the best way to chemically separate two optical isomers?

What is the best way to chemically separate two optical isomers?

I am trying to find a currently used method,  but any suggestions are welcomed. The optical isomers are of a metal complex. Thanks.

riza   422   1 Reply

tonih79

physio ex 9.0

physio ex 9.0

I am having a hard time with physioex 9.0. if someone can help that would be greatly appreciated.

johnvaughn   726   1 Reply

bio_man

PhysioEx 9.0 Exercise 6

PhysioEx 9.0 Exercise 6

Anyone can help?  I would appreciate it

PhysioEx 9.0  Exercise 6

Activity 5

1.  Describe the efffect that increasing the calcium ions had on the heart?

2.  Describe the effect that incre

lovethelight   1191   1 Reply

robertling

human population growth and theenvironment

human population growth and theenvironment

Describe historical pattern of growth of the worldwide human population since our origin.

hootie   1014   1 Reply

MSC88

Osmolarity of water in red blood cells?

Osmolarity of water in red blood cells?

I am confused on how osmolarity of cells with water would cause a cell to swell, shrink, or burst. I know that a cell has a solution concentration of 300 mosm. In a lab that was done, we analyzed the impact of a red blood cell in solutions containing

Violagirl   1641   1 Reply

bio_man

How does a protein in buffer stick to a plastic plate?

How does a protein in buffer stick to a plastic plate?

In order to stick antibodies on to a plastic plate, we put the antibodies in a buffer and let it sit overnight at 4 C. I was wondering what the mechanism of this adhesion is, does it reach equillibrium? Is it east to get off if you wanted to remove i

rivertube   346   1 Reply

rizzy81

discrepancy b/t lower and upper div courses

discrepancy b/t lower and upper div courses

why is it that all the stuff we learned in lower div courses are contradictory in upper div?

bobaholic   546   1 Reply

bio_man

Biological vs. Technical replicates and data processing, so confused!

Biological vs. Technical replicates and data processing, so confused!

How do you combine data from biological replicates that contain technical replicates into one set of data that represents an average for a repeated experiment with a n of > 3?

Example: Say I have cancer line X and drug Y. What I do:

CityD   996   1 Reply

colleen

Physioex9.0 help

Physioex9.0 help

Hello please help..exercise 7 in physioex9.0

 draw a spirogram that depicts a persons volumes and capacities before and during a significant cough?

Chart2 spirometry results

Suezan26   5386   1 Reply

bio_man

Use the following gene in protein synthesis:

Use the following gene in protein synthesis:

 Use the following gene in protein synthesis:
 TAGGACCATTTTAGCCCC
 o  Show the mRNA.
 o  Show the tRNA.
 o  Name the amino acids that will be placed in the polypeptide chain

help

domenicog   2773   1 Reply

duddy

Animal-Derived Laboratory Reagents

Animal-Derived Laboratory Reagents

I have a question about animal-derived laboratory reagents and products. I’m working in a strictly in-vitro lab that focuses mainly on western blots and flow cytometry. I was wondering the cost to animal life associated with these reagents. These inc

southtowns18   1074   1 Reply

lvnorbust

What goes to the negative side in gel electrophoresis?

What goes to the negative side in gel electrophoresis?

What type of substances in general would be attracted to the negative side?

smjsa79   624   1 Reply

nutjob2011

can you give me suggestion to increase efficiency and effectifity in my chromatography assay?

can you give me suggestion to increase efficiency and effectifity in my chromatography assay?

can you give me suggestion to increase efficiency and effectifity in my chromatography assay?

Illuminatus   516   1 Reply

tommytoughnut

Alternative method to pull down assay for protein-protein interactions?

Alternative method to pull down assay for protein-protein interactions?

I was wondering what other methods exist in addition to GSP-fusion/pull down assay and mass spec. to determine interaction of one protein with the other.  I won't be able to perform crystallography, so that is not an option.

Is there anything else?

illicitf8   645   1 Reply

micro_2012

How does the assay detect the level of activity??

How does the assay detect the level of activity??

How does the assay detect the level of activity??

ilndrz   466   1 Reply

LES

What is the difference between the Assay and Purity ?

What is the difference between the Assay and Purity ?

What is the difference between the Assay and Purity ?

smn   788   1 Reply

ilndrz

How to accurately do Protein/Enzyme Assay Calculations?

How to accurately do Protein/Enzyme Assay Calculations?

This is for my Biochemistry of Macromolecules Lab.....
For determining enzyme concentration (umole/min) for M1, P1, P2, and S2 using the fact that 9OD=1 umole, does these calculations seem reasonable.
The OD's are: M1=.888, P1=.352, P2=.473, and S2=

irina   5941   1 Reply

illicitf8

What is an assay and in particular a protein assay?

What is an assay and in particular a protein assay?

What is an assay and in particular a protein assay?

LESHA   736   1 Reply

Asinense

alcohol dehydrogenase assay, how do you know when things go wrong?

alcohol dehydrogenase assay, how do you know when things go wrong?

the actual question reads:
how would you know if the reaction preparation of a alcohol dehydrogenase has been compromised?

lerado1480t   461   1 Reply

smn

What is the principle of biuret method in protein assay and its purpose?

What is the principle of biuret method in protein assay and its purpose?

What is the principle of biuret method in protein assay and its purpose?

lerado1480t   1084   1 Reply

midkidd

How long does incubation take for chemistry assay's on the Abbott axiom?

How long does incubation take for chemistry assay's on the Abbott axiom?

How long does incubation take for chemistry assay's on the Abbott axiom?

tommynkell   472   1 Reply

tommy_gun

Can you explain me about the Lowry assay that use for determininng protein?

Can you explain me about the Lowry assay that use for determininng protein?

Can you explain me about the Lowry assay that use for determininng protein?

les4212   470   1 Reply

!JRon@n

what is yeast two-hybrid analysis and what is an overlay assay and what is a dot overlay assay?

what is yeast two-hybrid analysis and what is an overlay assay and what is a dot overlay assay?

please answer the whole question. thanks.

asinger96   713   1 Reply

smokebomb13

What is the equation of the reactions involved in the standardization of sulfuric acid and assay of NaHCO3?

What is the equation of the reactions involved in the standardization of sulfuric acid and assay of NaHCO3?

What is the equation of the reactions involved in the standardization of sulfuric acid and assay of NaHCO3?

datoledo88   2974   1 Reply

micronut

Will the MTT assay work in cells grown under hypoxic conditions?

Will the MTT assay work in cells grown under hypoxic conditions?

Will the MTT assay work in cells grown under hypoxic conditions?

lesab1   790   1 Reply

micronat

You does the color of the cell medium change from red to yellow, as in DMEM medium with 10% fetal bo

You does the color of the cell medium change from red to yellow, as in DMEM medium with 10% fetal bo

Y the color of the cell cul medium is changing from red to yellow Eg. DMEM medium with 10% fetal bovine serum?

rjframe   1169   1 Reply

buglitter

What is the concentration of the obtained DNA?

What is the concentration of the obtained DNA?

DNA Extraction protocol
(Procedure)


Method
Qiagen Easy DNA blood and Tissue Kit will be used.
1-Bring all reagents at room temperature
2-Prepare the reagents as mentioned in the handbook (done by the instructor)
3-Add 20 µl proteinase  K to the b

terminator   571   1 Reply

obb

in a fire assay for gold what is the best way to overcome the effect of ilmenite and iron in the crucible?

in a fire assay for gold what is the best way to overcome the effect of ilmenite and iron in the crucible?

in a fire assay for gold what is the best way to overcome the effect of ilmenite and iron in the crucible?

asing96   535   1 Reply

smokebomb13

Why use naproxen as internal standard in ketorolac injection assay?

Why use naproxen as internal standard in ketorolac injection assay?

Why use naproxen as internal standard in ketorolac injection assay?

tommynkell   531   1 Reply

midnight1

How does gel electrophoresis help determine relatedness in a species?

How does gel electrophoresis help determine relatedness in a species?

A. It matches mRNA.
 B. It shows mutations.
 C. It compares DNA.
 D. It predicts adaptations.

bug2012   2184   1 Reply

mickey27121

What permits gel electrophoresis to separate fragments of DNA?

What permits gel electrophoresis to separate fragments of DNA?

a.) Differeneces in the sizes of DNA fragments only.
b.) The number of the negative charges on the fragments only.
c.) The net charge (negative or positive) on the fragments only.
d.) The size and net charge (negative or positive) on the fragments.

bugbugs4   1217   1 Reply

NursingStud

Whats the difference between southern blotting analysis and Electrophoresis?

Whats the difference between southern blotting analysis and Electrophoresis?

Do they give different results?

flowe12   544   1 Reply

florrye

How do you explain the digestion in Gel Electrophoresis?

How do you explain the digestion in Gel Electrophoresis?

I need to do an essay for a lab and i wasnt there.

firas kilani   403   1 Reply

buffzyzz

How to read bands in agarose gel electrophoresis?

How to read bands in agarose gel electrophoresis?

I have a band that is between two bands of the ladder, how would I read that? For example one of my bands migrated to a position between 2000 and 1500 bp. Would I just say that the band that landed between those two ladder bands is 1750bp?

nursinghelp   1601   1 Reply

nursingneeded