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Imagine that it is 1965 and the bacterium Thermus aquaticus has not yet been discovered. As a ...

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Imagine that it is 1965 and the bacterium Thermus aquaticus has not yet been discovered. As a ...

Imagine that it is 1965 and the bacterium Thermus aquaticus has not yet been discovered. As a geneticist interested in forensics applications, you are attempting to develop a gene-amplifying technique similar to PCR. In a test tube, you place a DNA sample, free nucleotides, human DNA polymerase, and the appropriate primers for the DNA sequence being analyzed. You first heat the sample to 90°C, then cool it to 50°C, and then reheat it to 70°C.

Based on this scenario and your knowledge of how modern PCR works, the result of these procedures will be
A) successful copying of the DNA, because it follows modern PCR procedures almost perfectly.
B) unsuccessful, because the enzyme will denature during the first step (heating).
C) unsuccessful, because the enzyme will denature during the second step (cooling).
D) unsuccessful, because the enzyme will denature during the third step (reheating).
E) unsuccessful, because human DNA polymerase cannot recognize the primers used.

Textbook

Biology: Life on Earth

Edition: 10^th

Authors: Audesirk, Byers

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