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rinaann rinaann
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12 years ago
purify the chloroplasts from plants collected in the wild?

 A. Electron microscopy
   B. Freeze fracture
   C. Cell culture
   D. Cell fractionation
   E. Light microscopy

I'm just asking because I've looked everywere and I can't find the anwer. Thanks! Slight Smile
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12 years ago
D. Cell fractionation.

First you grind the plant material up with a pestle or a blender and add a mild detergent to break the cell membranes up.  Then you put them in a tube filled with a special sucrose mix that is more dense from top to bottom.  Put that tube in a centrifuge, and all the stuff that was inside the cells goes as far into the sucrose mix as their mass allows.  If you know what the weight of a chloroplast is, then you can drain that part out of the bottom of the tube.  If you don't know the weight, then you could drain the tube in sections and see which part is photochemically active.

PS. It's called cell fractionation because you drain the cell components from the bottom of the tube in "fractions" which each contain cell parts that weigh about the same.
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zulezule
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12 years ago
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