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Home Q & A Board Biology-Related Homework Help General Biology
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ownz ownz
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11 years ago
Ey,

Im doing a biology experiment for school to see what the affect is of the water PH on the biomass of the plant elodea. To explain this i took the enzym Ribusco and i know that its an important enzym in the calvin cyclus. But i wanted to ask you guys how does the PH EXACTLY affect ribusco. I found somewhere that the enzym will work slower which means that the enzym takes carbon oxide slower, which makes the photosynthesis process way slower.

btw sorry im not english, but i hope you understand this Face with Stuck-out Tongue
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#1
11 years ago
pH will denature the enzyme, by interacting with the atoms that make it up.

Quote
Upon illumination of the chloroplasts, the pH of the stroma rises from 7.0 to 8.0 because of the proton (hydrogen ion, H+) gradient created across the thylakoid membrane. At the same time, magnesium ions (Mg2+) move out of the thylakoids, increasing the concentration of magnesium in the stroma of the chloroplasts. RuBisCO has a high optimal pH (can be >9.0, depending on the magnesium ion concentration) and, thus, becomes "activated" by the addition of carbon dioxide and magnesium to the active sites as described above.

According to this, the enzyme needs a "basic" pH. Anything acidic would make it work less efficient. An  alkaline pH would probably promote photosynthetic activity.

I hope this explanation helps.
Would you know my name, if I saw you in heaven?

Eric Clapton
ownz Author
wrote...
#2
11 years ago
Quote from: nite_n_day (11 years ago)
pH will denature the enzyme, by interacting with the atoms that make it up.

Quote
Upon illumination of the chloroplasts, the pH of the stroma rises from 7.0 to 8.0 because of the proton (hydrogen ion, H+) gradient created across the thylakoid membrane. At the same time, magnesium ions (Mg2+) move out of the thylakoids, increasing the concentration of magnesium in the stroma of the chloroplasts. RuBisCO has a high optimal pH (can be >9.0, depending on the magnesium ion concentration) and, thus, becomes "activated" by the addition of carbon dioxide and magnesium to the active sites as described above.

According to this, the enzyme needs a "basic" pH. Anything acidic would make it work less efficient. An  alkaline pH would probably promote photosynthetic activity.

I hope this explanation helps.

Thanks for the answer, i finished the project and made an essay of it explaining how it exactly works. Anyways didnt know the the PH changes the structure of the enzym, so thanks for that.
wrote...
#3
11 years ago
You're welcome Smiling Face with Open Mouth

It either disrupts the structure or disrupts the active site, one or the other.
Would you know my name, if I saw you in heaven?

Eric Clapton
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