Experiment lifecycle

Are you wondering how to culture the MCF-7 cell line?

Just want to confirm before I answer your question.

no-no. that was just an example. 

Alright cool. So what is it that you want to be answered? lol

I think the question is...

Can anyone tell me about a biological experiment lifecycle?
I mean step by step - what is done, what is written. What tools are used to document the steps and results?
Do you use just Notepad or Word and Excel? 

The fact that you use the term life-cycle kind of throws me off . All experiments build off of previous knowledge and understanding of the topic being investigated. First, you must have a question, i.e. How do changes in substrate concentration, enzyme concentration, and environmental factors, such as temperature and pH, affect the rate of enzyme activity? Then you form a hypothesis (which is an educated guess based on previous knowledge. You then make predictions, i.e. you predict the effect that a change in temperature will have on the rate of catalase activity. You next conduct the experiment using some sort of experimental design, record your results, analyze your results,  evaluate your results for validity, and finally communicate your results by writing down what it means as a discussion or conclusion.

In science, a hypothesis is a tentative answer to a well-framed question-an explanation on trial. It is usually an educated guess, based on experience and on the data available from discovery science. A scientific hypothesis leads to predictions that can be tested by making additional observations or by performing experiments.