how does NaCl affect catalase

Every enzyme has an optimal salt concentration in which it can catalyze reactions. Too high or too low a salt concentration will denature the enzyme. In living systems, the optimal ranges of temperature, pH and salt concentration for a given enzyme are the ranges found in that system. When determining various optimum conditions for a catalase solution it is important to consider the source of the catalase. Catalase derived from a potato or from yeast might “prefer” slightly different condition than catalase derived from beef liver. For example, mammalian enzymes have a temperature optimum of about 40°C, but there are enzymes that work best at very different temperatures, e.g. enzymes from the arctic snow flea work at -10°C, and enzymes from thermophilic bacteria work at 90°C. The optimal pH range is about 7-8 (physiological pH of most cells), but a few enzymes can work at extreme pH, such as protease enzymes in animal stomachs, which have an optimum of pH 1.