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Home Q & A Board Biology-Related Homework Help Cell Biology
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msitaly83 msitaly83
wrote...
13 years ago
Microarray analysis using DNA Chips and Southern blot analysis both rely on a fundamental property of DNA that is the basis for their utility.  What is that property and why does this property cause temperature to be a critical factor in the performance of these types of analyses?
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wrote...
#1
Educator
13 years ago
I think the property they are referring to is the fact that the two strands of DNA are held together by hydrogen bonds. The more hydrogen bonds there are, the higher the amount of heat is required to separate the complimentary strands.

http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120078/micro50.swf::Microarray

http://highered.mcgraw-hill.com/olcweb/cgi/pluginpop.cgi?it=swf::535::535::/sites/dl/free/0072437316/120078/bio_g.swf::Southern%20Blot
msitaly83 Author
wrote...
#2
13 years ago
Thank you! The videos were very helpful in understanding how the microarrays and Southern Blotting work.
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