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queenhoa queenhoa
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10 years ago
Can anyone please help me with these lab questions?
These are questions from the lab called: Identification of Serum Glcoprotein by Polyacrylamide Gel Electrophoresis and Albumin Western Blotting.

1. Describe the results of your Coomasie- stained gel picture. What is in each lane?  Do you have multiple bands or single bands? Why?

2. Which lanes had bands in the sample Western Blot that your TA showed you from this experiment? Why?

3. You accidently use an SDS-PAGE system to do this experiment. What impact would this have on your Western blot results?


4. If you wanted to determine the molecular weight of albulmin, how would you change your experimental protocol?


Attached is my gel picture. Please help me identify which bands that most likely corresponds to serum albumin protein.

The reason I'm asking these questions is because my TA grades very tough and is very specific in what she wants even when I give good answers. Your opinions will be very helpful to me. Thank you.
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robertlingrobertling
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10 years ago
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queenhoa Author
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#2
10 years ago
Thank you robertling.
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#3
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10 years ago
Quote from: queenhoa (10 years ago)
Thank you robertling.

My pleasure
Don't forget to give me a thumbs up!
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#4
10 years ago
thanks robertling
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#5
10 years ago
thanks robert
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#6
10 years ago
Thanks Robert!!!
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#7
10 years ago
Thank you robert
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#8
10 years ago
I was so desperate
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#9
10 years ago
Quote from: robertling (10 years ago)
Quote from: queenhoa (10 years ago)
3. You accidently use an SDS-PAGE system to do this experiment. What impact would this have on your Western blot results?

I have a really good lecture that describes the difference between the two.
Thank you Robert.
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#10
10 years ago
Thanks
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#11
10 years ago
Thank you so much!!!
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#12
10 years ago
Thanks robertling!
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#13
10 years ago
Quote from: robertling (10 years ago)
Quote from: queenhoa (10 years ago)
3. You accidently use an SDS-PAGE system to do this experiment. What impact would this have on your Western blot results?

I have a really good lecture that describes the difference between the two.

 thanks roberting
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