Ca-phosphate transfection method

CaPO4 - co-precipitation involves precipitating the dna with calcium by increasing the ability of the cell to uptake calcium with dna.
Steps:
assuming we have a virus with its viral dna
add phosphate buffer and calciumchloride.
dna precipitates as cells are uptaking calcium for their signalling pathways. as it is taking the calcium, it is also uptaking dna as it is precipitated with the calcium
the dna-ca precipitate in the cell is incubated at 37 degrees for some time and then selected for after transfection has taken place.
an infection into a mammalian cell without lysis by budding out of the cell, producing new viruses to propogate.
for example, lukemia cells transfected with the human t-lymphotropic virus