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albania94 albania94
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9 years ago
Provide an explanation for the high absorbance reading in the 80-degree control tube in the Temperature assay.
Discuss the effect of high temperature dependence as potential sources of error in the measure of enzymatic activity.
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9 years ago
Each enzyme has an optimum temperature at which it works best. A higher temperature generally results in an increase in enzyme activity. As the temperature increases, molecular motion increases resulting in more molecular collisions. If, however, the temperature rises above a certain point, the heat will denature (def) the enzyme, causing it to lose its three-dimensional functional shape by denaturing its hydrogen bonds. Cold temperature, on the other hand, slows down enzyme activity by decreasing molecular motion.
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