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yetiintheback yetiintheback
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9 years ago
Typical Bio lab... We transformed e.coli to having the pGreen gene and glowing in the dark. You'll see on the photo the first column we used a MWS and the second column was the pGreen +ampicillin mixture and we were able to isolate the gene to show up. But on the third column it was the pGreen mixture but no ampicllin, so why didn't this isolate the gene so we could see it? Thanks!
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rsb
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9 years ago
Quote from: yetiintheback (9 years ago)
But on the third column it was the pGreen mixture but no ampicllin, so why didn't this isolate the gene so we could see it? Thanks!

If it wasn't done correctly, it won't be visible. I've run into this problem several times before. Frowning Face
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