Thank you for all the help

I'm getting sooo frustrated with this exercise. I have finally found the answers to the actual activites but can't find anything for the review sheet without buying them.

Ask the questions you need help on, what exercise?

On Exercise 3. Q: Did the pepsin or deionized water contain and contaminating digested BAPNA? Which tubes confirm this?

Would it be that pepsin did and deionized water did not. Because tubes 2 and 5 did not have an optical density of 0.00? That was my first thought, but then tube 1 DID have and optical density of 0.00 and it had the same substances at tube 2.

Also on exercise 4...Q: Can you determine whether fat hydrolysis has occurred in tube 6? Explain your answer.

Would it be yes just because of the fact that the pH after incubation was different than the pH buffer added to the test tube

Activity 4: Assessing Fat Digestion by Pancreatic Lipase and the Action of Bile (pp. 109–111)

7. Tube 1 investigated the action of bile on enzyme activity, and tube 2 examined lipase activity without bile. Bile enhances fat digestion by lipase. Yes, you can determine if activity occurred in tube 6 because the pH would drop below pH 9.0. A small amount of fat digestion occurred because pH decreased from 9.0 to 8.97. The optimim pH for lipase activity was pH 7.0. Using a pH method to assay for activity at pH 2.0 does not work because the buffer is already quite acidic. There could have been activity at pH 2.0 that was not detectable by this method. In theory, lipase would be active in the mouth because its pH optimum is relatively neutral. However, it would not be active in the stomach because of the acidic pH condition. The substrate is vegetable oil (fat). The subunit formed is fatty acid (and monoglycerides).

I need help please with exercise 8 activity 3 and 4

Start a new topic and I'll help!

Having difficulty getting the links to work....any ideas?

When our class did this lab, this is what we were supposed to understand:

Pepsin and acid are more optimal for enzyme action - The acid helped the pepsin act more effeceintly. Boiling the test tubes sped up the process and allowed for a quicker reaction (the Benedict solution began changing color). That change in color was evidence of glucose.

I need help with A&P 8.0 Exercise 8 review questions..thx

Could you post the question?

no abrio el enlace que mandaron

This thread has helped a bunch, thank you so much!