Thanks, that really helped!

thx for your help in this...this was a hard lab

no work:(

What do tubes 2, 6 and 7 reveal about pH and amylase activity?-pH has a strong affect on amylase activity.

Which pH buffer allowed the highest amylase activity?-7.0

Which tube indicates that the amylase did not contain maltose?-3

4.If control tubes were left out, what objections could be raised against the statement "Amylase digests maltose to starch"?
   Contamination of maltose or starch would yield a false positive result.

5.Why would the amylase present in the saliva not be active in the stomach?
   The pH in the stomach is too low.

6.    What effect does boiling have on enzyme activity?
   Boiling inactivates enzymes.

7.   Refer to the data you collected for Activity 2: Assessing Cellulose Digestion. Which tubes showed a positive test for the IKI reagent?
   Tubes 4, 5, and 6

8.Which tubes showed a positive test for the Benedict's reagent?
   Tubes 1, 2, 3, and 7

9.What was the effect of freezing tube 1?   
   Freezing had no effect.

10.How does the effect of freezing differ from the effect of boiling?
   Freezing has no effect on enzyme activity.

11.Which of the following explanations best describes the effect of amylase on glucose in tube 3?
   Amylase had no effect on the glucose.

12.What was the effect of amylase on cellulose in tube 4?
      Amylase had no effect on cellulose.

13. Judging from the results of tubes 4, 5, and 7, what can be concluded about the digestion of cellulose?
   Salivary amylase does not digest cellulose.

14.   What was the effect of peptidase in tube 6?
   Peptidase had no effect because it doesn't work on carbohydrate substrates.

15.   Refer to Activity 3: Assessing Protein Digestion by Pepsin. Which pH provided the highest pepsin activity?
   2.0

16.Tubes 1 and 2 contained the same substances. Why were their optical density measurements different?
      The contents of tube 1 were boiled, which inactivated the pepsin; therefore there was no activity in tube 1.

17.What would happen if the temperature were decreased to 10°C?
   The amount of BAPNA digested would be less.

18.   Refer to Activity 4: Assessing Fat Digestion by Pancreatic Lipase and the Action of Bile. What was the difference in activity between test tubes 1 and 2?
   Tube 1 had greater activity because of the presence of bile.

19   Which pH resulted in maximum lipase activity?
      7.0

20.    In theory, would lipase be active in the mouth?
   Yes, in theory lipase would be active in the mouth because the pH optimum is relatively neutral.

This thread is so helpful with this exercise!

link doesnt work

it does for me.

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Thanks for the posts

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Thanks for the upload!

Thank you very much