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DLS DLS
wrote...
Posts: 30
Rep: 8 0
8 years ago
A mutant mammalian gene has a premature stop codon but a Western blot detects no truncated protein. A plasmid is cloned from the wild-type gene and the mutant gene's premature stop codon is inserted at the same position. After transfecting the mutant plasmid into the mutant gene, the Western blot now detects the truncated protein.  Why?

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