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hellohi hellohi
wrote...
Posts: 195
Rep: 5 0
13 years ago
Hi everyone! Waving Hand Sign I was wondering if I could get some help for my biology project.

We have to pick an enzyme and pick a topic.

I chose protease as my enzyme and Induced-fit model of an enzyme-substrate interaction.

I have to give an explanation and a model that anyone in my class would be able to understand.

Could you guys help me start? My partners fairly busy this weekend and I gotta do this alone, so please help:)

Thanks everyone Smiling Face with Open Mouth
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32 Replies
Replies
hellohi Author
wrote...
13 years ago
Edit: Actually could you guys give me a list of enzymes I could use?? I need to pick a specfic metabolic enzyme
hellohi Author
wrote...
13 years ago
Okay everyone, I think im gonna do dehydrogenase! Can you guys help me start?!?! TY:)!
hellohi Author
wrote...
13 years ago
Maybe this will help you help me:    


I chose number 10

http://en.wikipedia.org/wiki/Citric_acid_cycle 
wrote...
On Hiatus
13 years ago
You know what.  I had the exact same project when I was in Grade 12, except our teacher assigned a macromolecule to each of the groups and my group was given carbohydrates. 

Btw, there were two seperate groups assigned in my class as proteins and enzymes. 

If only I could remember what the enzymes group did in their presentation... Face with Rolling Eyes

If I think of something unique, I will get back to you.  Smiling Face with Open Mouth
hellohi Author
wrote...
13 years ago
Thank you! You've given me hope:)! Ive done a little research myself but could you tell me, the enzyme-substrate interaction that malate dehydrogenase is in?? I just need to understand it O_o Thanks:)!

hellohi Author
wrote...
13 years ago
P.S, did you have to explain that as well?
wrote...
On Hiatus
13 years ago
P.S, did you have to explain that as well?

Actually, the presentation was not on a specific enzyme.  It was on enyzmes in general and what goes on in an interaction between an enzyme and a substrate.  Smiling Face with Open Mouth 
hellohi Author
wrote...
13 years ago
Oh:( could you help me with the written part at least,

2. Induced-fit model of an enzyme-substrate interaction.
wrote...
On Hiatus
13 years ago
The Induced fit model describes the formation of the E-S as a result of the interaction between the substrate and a flexible active site on the enzyme.  The substrate produces changes in the conformation on the enzyme, aligning properly the groups in the enzyme.  It allows  better binding and catalytic effects. 



This model opposes to the former Lock and Key model, that explains the formation of the E-S complex as a result of the binding of complementary geometrical rigid structures, as a lock and a key.

Here's an animation that represents the two models:

http://www.wiley.com/legacy/college/boyer/0470003790/animations/enzyme_binding/enzyme_binding.htm

Hope that helps!  Smiling Face with Open Mouth


hellohi Author
wrote...
13 years ago
Thank you for your help, I have a question. You know how I'm doing malate dehydrogenase, could I use that model you showed me as my project? how would I do It? Thanks:)!
wrote...
On Hiatus
13 years ago Edited: 13 years ago, Bio_World100
Thank you for your help, I have a question. You know how I'm doing malate dehydrogenase, could I use that model you showed me as my project? how would I do It? Thanks:)!

On the enzyme, you can make a textbox and write in "dehydrogenase" and for the substrate, you can do the same and write in "malate".  You don't need to make two seperate shapes for the substrate like in the image I posted.  It can be any shape.  Also, draw an arrow pointing to the active site. 

Malate dehydrogenase catalyzes the oxidation of the ?-hydroxy group of malate to a keto group, forming oxaloacetate.  The enzyme alongside reduces NAD+ to NADH.  So, what you could do is add the malate-dehydrogenase interaction where it says malate dehydrogenase in the following image: 
 


Btw, the dashed lines in those two structures represent resonance. 

Hope this helps!  Smiling Face with Open Mouth
hellohi Author
wrote...
13 years ago
My project needs a 3d model, I'm thinking of using lego peices. Heres the thing; I'm thinking of making a big box lego and leaving some space in it to put the "enzyme". So the active site will be the lego cube im gonna make and I'll leave 2 places for the substrate to attach. When it comes out, the color will change of the substrate. I don't know what else to think of tho.
hellohi Author
wrote...
13 years ago
Btw, what would i label the NAD+ as and stuff like that? I have the basic concept but how would I relate it to the actual reaction? Thanks for your help:)!!
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